| 摘要: |
| [目的]研究家兔BMP7基因3'非翻译区(3'UTR)序列的结构及功能特点.[方法]以家兔肾脏组织为材料,应用3'RACE技术对家兔BMP7基因3'UTR序列进行扩增,并与GenBank中登陆的其他哺乳动物相应序列进行比较分析.[结果]成功克隆了家兔BMP7基因的3'UTR(GenBank号:EU004072);在3'UTR序列末端存在2个切割与胞质多聚腺苷化特异因子(CPSF)结合位点(AAUAAA)和1个11 bp的poly(A).家兔BMP7基因3'UTR序列与小鼠、人、牛和猪的一致性分别为41.45%,50.30%,47.84%和57.91%,相对不保守.[结论]家兔BMP7基因3'UTR结构特点可能与其基因功能有关. |
| 关键词: 家兔 哺乳动物 骨形态形成蛋白7 生物信息学分析 |
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| 基金项目:河南省教育厅自然科学基金，河南省科技攻关项目? |
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| Cloning and sequence analysis of the 3'UTR of rabbit BMP7 |
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| Abstract: |
| 【Objective】 The recearch investigated the structural and functional characteristics of 3′untranslated region (3'UTR) of rabbit bone morphological protein 7 gene (BMP7).【Method】 Taking the domestic rabbit kidney organization as the material,3′UTR of rabbit BMP7gene was cloned by RACE method,then comparative analysis was conducted using bioinformation methods. 【Result】 The sequence of 3′UTR of rabbit BMP7gene was cloned firstly (Genbank accepted number is EU004072).There were 2 binding sites of cleavage and ployadenylation factor specificity factor (CPSF) and a poly A tail (11 bases at length).The 3′UTR of rabbit was 41.45%, 50.30%, 47.84% and 57.91% identical to those of mouse,human,cow and pig respectively.【Conclusion】 The structure of 3′UTR of rabbit BMP7 gene may be related to the function of the gene. |
| Key words: rabbit mammalian BMP7 3′UTR bioinformation analysis |
