| 摘要: |
| [目的]分析灰斑古毒蛾核型多角体病毒(Orgyia ericae nucleopolyhedrovirus,OrerNPV)EcDR Ⅰ-U片段分子生物学特征,为研究其分子特性及其在OrerNPV感染周期中的功能提供科学依据.[方法]克隆、分析Or-erNPV EcoR Ⅰ-U片段所包含的若干基因,并应用生物软件对OrerNPV泛素基因(Ubi基因)进行了进化分析.[结果] OrerNPV EcoR Ⅰ-U片段包含Ubi基因、AcORF34同源基因和39K基因部分序列,在该区域中编码Ubi基因的开放阅读框(ORF)由246个核苷酸组成,可以编码81个氨基酸,预计蛋白质分子质量为8.9 ku.OrerNPV Ubi基因与苜蓿丫纹夜蛾核型多角体病毒(AcMNPV)、家蚕核型多角体病毒(BmNPV)、薄荷灰夜蛾核型多角体病毒(RaouM-NPV)的同源性最高,均达92%,与小菜蛾颗粒体病毒(Plutella xylostella granulovirus, PxGV)的同源性最低,为62%.OrerNPV与AcMNPV、BmNPV和RaouMNPV的亲源关系最近.EcoR Ⅰ-U片段+M13端含有编码39K基因(pp31)的部分序列,该多肽与10种NPV的39K序列有48%～52%的同源性.[结论]明确了OrerNPV EcoR Ⅰ-U片段的序列特征及OrerNPV Ubi在泛素蛋白家族中的进化关系. |
| 关键词: 灰斑古毒蛾核型多角体病毒 EcoRⅠ-U片段 泛素基因 AcORF34同源基因 39K基因 分子进化 |
| DOI： |
| 分类号:S432.1 |
| 基金项目:河南省基础与前沿项目，河南省农业科学院博士启动项目? |
|
| Analysis of EcoR Ⅰ-U fragment from Orgyia ericae Nucleopolyhedrovirus |
|
YANG Li-rong,XUE Bao-guo,LIU Hong-yan,WANG Zheng-jun,WANG Xiu-mei,YANG Li-rong,XUE Bao-guo,LIU Hong-yan,WANG Zheng-jun,WANG Xiu-mei,YANG Li-rong,XUE Bao-guo,LIU Hong-yan,WANG Zheng-jun,WANG Xiu-mei
|
| Abstract: |
| 【Objective】 Molecular characteristics were analyzed on Orgyia-ericae-nucleopolyhedrovirus（OrerNPV）.【Method】 Here, the cloned OrerNPV Eco RⅠ-U fragment in which the Ubi and homologues of AcORF34 and 39K were found was analyzed. 【Result】 The Ubi open reading frame was 246 nucleotides long that coded a peptide of 81 amino acids with a predicted molecular weight of 8.9 ku.The peptide of OrerNPV Ubi shared a highest similarity of 92% to Autographa californica multiple nucleocapsid nucleopolyhedrovirus,Bombyx mori nucleopolyhedrovirus and Rachiplusia ou multiple nucleocapsid nucleopolyhedrovirus;while it shared a lowest similarity with Plutella xylostella granulovirus GV of 62％.The phylogenetic results of Ubi based on 21 species showed that AcMNPV,BmNPV and RaouMNPV were mostly related to OrerNPV.The 39K amino acid residues in the M13+ terminal of OrerNPV had 48％-52％ identities with homologous genes from other 10 NPVs.【Conclusion】 The result clarified the characteristics of OrerNPV Eco RⅠ-U fragment and phylogenetic relationships of OrerNPV Ubi gene in the Ubi family. |
| Key words: Orgyia ericae nucleopolyhedrovirus Eco RⅠ-U Fragment Ubi gene homologues of AcORF34 39 K gene molecular evolution |
