| 摘要: |
| [目的]探讨S-100蛋白、5-羟色胺(5-HT)、神经特异性烯醇化酶(NSE)和突触素(SYP)4种弥散性神经内分泌细胞标志物在黄体细胞中的共存情况.[方法]采取妊娠中期(50～70 d)奶山羊卵巢,常规方法制备石蜡切片,采用免疫荧光双标记法结合激光扫描共聚焦显微镜观察,对4种弥散性神经内分泌细胞标志物的分布进行了检测.[结果]S-100蛋白与SYP共存于同一黄体细胞中,表达呈强阳性,阳性细胞主要分布在黄体周边,占总阳性细胞的56.57%.S-100蛋白与5-HT、5-HT与SYP、NSE与SYP、5-HT与NSE、S-100蛋白与NSE共存的细胞呈弱阳性,分别占总阳性细胞的18.42%,15.67%,4.31%,4.30%和0.73%,均匀分布于整个黄体中.[结论]S-100蛋白、5-HT、NSE和SYP在山羊黄体细胞中有两两共存现象. |
| 关键词: 神经内分泌标志物 免疫荧光双标记 激光扫描共聚焦显微镜 黄体 山羊 |
| DOI： |
| 分类号:S827.1 |
| 基金项目:国家自然科学基金，陕西省自然科学基金? |
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| The coexistence of diffused neuroendocrine system cell markers in corpus luteum cells of pregency goat |
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CHEN Shu-lin DUAN Hui-juan ZHANG Bao LEI Zhi-hai JIANG Tian-yuan LIN Lei LI Zhao-cai MA Yong-sheng
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| Abstract: |
| 【Objective】 The study investigated the coexistence of four diffused neuroendocrine markers in the corpus luteum cells,including S-100 protein,5-hydroxytryptamine (5-HT),neuron specific enolase （NSE） and synaptophysin （SYP）.【Method】 Four diffused neuroendocrine markers in dairy goat ovary slice during mid trimester of pregnancy（50-70 d） were determined by double immunofluorescence labeling for confocal laser scanning microscopy.【Result】 Coexpression of S-100 protein and SYP expressed in the same corpus luteum cell and expression was strongly positive,which distributed in the perimeter of the corpus luteum.The positive cells coexpressing S-100 protein and SYP accounted for 56.57% of the total positive cells.Coexistence of S-100 protein and 5-HT,5-HT and SYP,NSE and SYP,5-HT and NSE,S-100 protein and NSE were weakly positive.They accounted for 18.42%,15.67%,4.31%,4.30% and 0.73% of the total positive cells respectively,and distributed uniformly in corpus luteum.【Conclusion】 Coexistence between four diffused neuroendocrine markers in the corpus luteum cells of dairy goat was presented. |
| Key words: neuroendorine marker double immunofluorescent labeling confocal laser scanning microscope corpus luteum goat |
