| 摘要: |
| [目的]探讨陕北白绒山羊激素敏感脂酶(Hormone sensitive lipase,HSL)基因外显子1的SNP遗传多态性及其与产绒、胴体和生长性状的相关关系,为陕北白绒山羊分子标记辅助选择提供理论依据.[方法]测定168只陕北白绒山羊部分生长、胴体指标.利用PCR-SSCP技术分析HSL基因外显子1的SNP遗传多态性.[结果]陕北白绒山羊HSL基因外显子1有AB和AA 2种基因型,在群体中处于Hardy-Weinberg平衡状态;AB与AA基因型个体的绒长差异极显著(P<0.01),体高和背膘厚差异显著(P<0.05),其他性状均无显著差异;AB基因型个体初生重、断奶重、绒长、周岁重、体高、体长、管围、背膘厚大干AA基因型,但AA基因型个体毛长、产绒量、胸围、眼肌面积大于AB基因型.[结论]陕北白绒山羊HSL基因外显子1具有多态性,该基因可能是陕北白绒山羊产绒及胴体性状的主效基因,或者与控制这些性状的主效基因相连锁. |
| 关键词: 陕北白绒山羊 HSL基因外显子1 单核苷酸多态性 |
| DOI： |
| 分类号:S827.2 |
| 基金项目:教育部新世纪优秀人才支持计划? |
|
| Study on SNPs of exon 1 of HSL gene and its relationship with several production traits of Shaanbei white cashmere goats |
|
LIU Jun-xia LUO Jun TENG Yan-ling SHENG He-jun LIN Xian-zi YU Gang
|
| Abstract: |
| 【Objective】 This research analyzed the single nucleotide polymorphisms (SNPs) in the exon 1 of HSL gene and its relationship with some growth and carcass traits in Shaanbei White-Cashmere Goat in order to provide a theoretical foundation for the marker-assisted selection.【Method】 Several growth and carcass traits of 168 Shaanbei white cashmere goats were measured in the study.PCR-SSCP technique was used to analyze the SNPs at the exon 1 of the HSL gene. 【Result】 The exon 1 of HSL gene displayed AA and AB genotypes and the population was at the Hardy Weinberg equilibrium.There was a significant difference between the genotype AB and AA for cashmere length (P<0.01),wither height and back fat thickness (P<0.05).There was no significant difference for other traits.The trend of “AB>AA” was noted for birth weight,weaning weight,cashmere length, yearling weight,wither height,body length,circumference of cannon and back-fat thickness.The trend of “AA>AB” was observed in the traits of hair length,cashmere yield,heart girth and loin eye area.【Conclusion】 The exon 1 of HSL gene of Shaanbei white cashmere goat demonstrated polymorphism.It suggested that HSL gene might be a major gene or a major gene-linked gene for growth and carcass traits of Shaanbei white cashmere goats. |
| Key words: Shaanbei white cashmere goat Exon 1 of HSL gene PCR-SSCP SNPs |
