| 摘要: |
| 【目的】为新型抗生素的开发研究提供有效途径。【方法】对放线菌SL-5在链霉素不同浓度下的耐药突变菌株进行分离和活性筛选,并采用单因素和正交试验对活性菌株进行发酵优化。【结果】从无活性放线菌SL-5的耐药菌株中得到1株活性突变菌株str-14,室内生测结果表明,其发酵液对蜡状芽孢杆菌(Bacillus cereus)的抑菌圈直径为17.5 mm。通过单因素和正交试验,确定该菌株的最佳摇瓶发酵培养基配方为:小米10 g/L,葡萄糖20g/L,酵母粉2 g/L,蛋白胨7 g/L,混合VB1.5 mg/L,NaCl 2.5 g/L,CaCO32.0 g/L。最佳培养条件为:初始pH为8.0,装液量70 mL(250 mL三角瓶),180 r/min培养7 d。【结论】通过引入链霉素耐药性突变获得抑菌活性物质,是创制新型抗生素的一条可行之路。 |
| 关键词: 放线菌 链霉素耐药突变菌株 分离 发酵优化 |
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| 基金项目:国家“973”项目“从天然生物源小分子发现农用新作用靶标机制及先导结构”（2003CB114404） |
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| Studies on the isolation,activity screening and the fermentation optimization of streptomycin-resistance mutants of actinomycete SL-5 |
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| Abstract: |
| 【Objective】 The studies were carried out to provide an efficient way of discovering novel antibiotics.【Method】 Mutants were isolated at different streptomycin concentrations from wild strain SL-5 and under bioactivity screening,then the fermentation medium optimization of the active mutant was studied by the single-factor and orthogonal test.【Result】 Strain str-14 was obtained from the streptomycin-resistance mutants of inactive strain SL-5，and the result of bioassay in vitro showed that the fermentation broth of str-14 exhibited obvious inhibition circle of 17.5 mm agains (Bacillus cereus).The results of single-factor and orthogonal test showed that the optimum fermentation of strain str-14 for producing the most effective fermentation product were Millet 10 g/L,Glucose 20 g/L,Yeast extract 2 g/L,Peptone 7 g/L,Compound vitamins B 1.5 mg/L,NaCl 2.5 g/L，CaCO3 2.0 g/L.The optimum conditions were initial pH of 8.0，media volume 70 mL,shaking at 180 r/min for 7 days.【Conclusion】 It is a feasible way for the discovery of new antibiotics to find active substance by inducing streptomycin-resistance mutation. |
| Key words: actinomycete streptomycin-resistance mutant isolation fermentation optimization |
