| 摘要: |
| 【目的】研究控制小麦株高的数量性状位点(QTL)。【方法】利用SSR和AFLP分子标记构建连锁图谱,在3种不同试验环境(2003～2004年北京、2004～2005年北京和河南安阳)下分析百农64×京双16组合的218个F2:3株系群体的株高。【结果】构建了由158个分子标记(100个SSR标记和58个AFLP标记)位点组成的遗传连锁图谱,覆盖了除1D连锁群外小麦全基因组的3 114 cM;检测到3个控制株高的QTL,分别位于2B、4D和6A染色体上,贡献率分别为7.3%～11.5%,7.4%～12.9%和5.7%～11.3%。【结论】3个株高QTL位点在不同环境下表现稳定,其紧密连锁的分子标记可用于矮秆、半矮秆小麦的标记辅助育种。 |
| 关键词: 普通小麦 株高 数量性状位点(QTL) SSR标记 AFLP标记 |
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| 基金项目:国家“863”计划项目（2006AA100102）；西北农林科技大学博士科研启动项目（01140501） |
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| QTL mapping for plant height of wheat using a F2:3 population |
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| Abstract: |
| 【Objective】 The research was done in order to study QTLs controlling plant height in wheat.【Method】 A population with 218 F2:3 lines derived from the cross between Bainong 64 and Jingshuang 16 was detected using SSR and AFLP markers in three environments,i.e.Beijing（2003-2004），Beijing and Anyang (2004-2005).【Result】 The results showed that a linkage map with 100 SSR and 58 AFLP markers,covering 3 114 cM of the wheat genome except chromosome 1D,was constructed.Three QTLs for plant height were found on chromosomes 2B,4D and 6A,which accounted for 7.3%-11.5%，7.4％-12.9% and 5.7%-11.3% of phenotypic variance respectively.【Conclusion】 These QTLs showed stable effects across three environments,and thus their closely linked molecular markers could be useful in marker-assisted-selection programs for breeding dwarf and semi-dwarf wheat cultivars. |
| Key words: common wheat (Triticum aestivum L.) plant height quantitative trait locus (QTL) SSR marker AFLP marker |
