| 摘要: |
| 【目的】为培育高抗逆的作物品种提供科学依据。【方法】以耐盐苜蓿“中苜1号”为外植体,采用根癌农杆菌(Agrobacterium tumefaciens)介导的叶盘法转化苜蓿。【结果】成功构建了含有半胱氨酸蛋白酶基因MsCP1和报告基因GUS的植物表达载体pCAMBLA1391-CP-GUS,共获得47株抗性再生植株。经PCR和RT-PCR检测,表明外源基因已成功整合到苜蓿基因组中,并在转录水平得到表达。【结论】初步获得了高抗逆的转基因苜蓿新品系。 |
| 关键词: 半胱氨酸蛋白酶 载体构建 转基因植株 紫花苜蓿 |
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| 基金项目:中国农业科学院北京畜牧兽医研究所基本科研业务费专项基金（ywf-td-3） |
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| Construction of plant expression vectors containing MsCP1 genes and preliminary study of transgenic alfalfa |
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| Abstract: |
| 【Objective】This study was done in order to give new insights into the breeding of high stress resistant crop cultivars.【Method】 The genetic transformation of alfalfa cultivars "Zhongmu No.1" mediated by Agrobacterim tumefaciens was studied by using the transient expression of GUS gene. 【Result】 A new plant expression vector pCAMBLA1391-CP-GUS,containing cysteine protease gene MsCP1 and GUS gene,was successfully constructed through sequential restriction digests and ligations recombination.47 transformation plants were obtained.PCR and RT-PCR analysis showed that the foreign MsCP1 and GUS gene were inserted into the genome of these plants and expressed in mRNA level. 【Conclusion】 New strains of transgenic alfalfa with high stress-resistance were primarily gained. |
| Key words: cysteine protease construction of plant expression vector transgenic plant alfalfa (Medicago sativaL.) |
