| 摘要: |
| 【目的】研究β-巯基乙醇(β-ME)对牛卵母细胞核成熟和孤雌胚胎发育的影响。【方法】向OMM和SOF中添加0,0.05,0.10和0.15 mmol/Lβ-ME,并在牛卵母细胞激活后,分别于1-细胞期、8～16细胞期、桑葚胚期向SOF中添加0.1 mmol/Lβ-ME,通过比较胚胎体外发育水平确定添加β-ME的最佳时期。【结果】β-ME对牛卵母细胞核成熟无显著影响(P>0.05);0.05和0.10 mmol/Lβ-ME均可显著提高牛孤雌胚胎的卵裂率及囊胚发育水平,但以0.10 mmol/Lβ-ME添加组的效果较佳;牛孤雌胚胎发育到1-细胞期和8～16细胞期前,添加0.10 mmol/L的β-ME,均可显著提高囊胚的发育水平(P<0.05),但1-细胞期添加β-ME时的囊胚率、囊胚细胞数显著高于8～16细胞期组(P<0.05),故1-细胞期是添加β-ME的最佳时机。【结论】β-ME对牛卵母细胞核成熟无促进作用,但可提高牛孤雌胚胎的卵裂率并促使囊胚发育,且在8～16细胞期前添加β-ME均可提高囊胚发育水平,但以1-细胞添加0.10mmol/Lβ-ME的促进效果最佳。 |
| 关键词: β-巯基乙醇 孤雌激活 卵母细胞 胚胎培养 牛 |
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| 基金项目:国家“863”高技术研究计划项目 (2004AA213072)；浙江理工大学科研启动基金项目（0716685-Y） |
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| Effect of β-mercaptoethanol on the development of the bovine parthenogenetic embryos |
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| Abstract: |
| 【Objective】 The study investigated the effects of β-Mercaptoethanol (β-ME) on bovine oocytes nuclear maturation and the development of parthenogenetic embryo. 【Method】 β-ME (0,0.05,0.10 and 0.15mmol/L,treatments) was added in the media OMM and SOF respectively.Simultaneously,just as the matured oocytes were activated,β-ME was added in to SOF at the 1 cell-stage,8-16 cell-stage and morula stage so that the rate of embryo development was compared. 【Result】 The results indicated that nuclear maturation was not affected significantly by the absence or presence of β-ME（P>0.05）;0.05 and 0.10mmol/L β-ME improved cleaved rates and development of blastocytes significantly,but the latter group was better.Before bovine parthenogenetic embryo progressed to 8-16 cell-stage,β-ME could significantly improve blastocyte development（P<0.05）,but the rate and the number of blastocytes were significantly different between 1-cell-stage group and 8-16 cell-stage group,therefore,it was the best time for addition of β-ME to embryo culture medium at 1-cell-stage. 【Conclusion】 The results indicated that the addition of β-ME in the media OMM and SOF had no effect on the nucear maturation，but β-ME improved cleaved rates and development of blastocytes significantly.Before bovine parthenogenetic embryo progressed to 8-16 cell-stage, β-ME could significantly improve the development of blastocyte. |
| Key words: β-mercaptoethanol (β-ME) parthenogenetic activation oocyte embryo culture bovine |
