| 摘要: |
| 牟瑞营 |
| 关键词: 奶牛 胎衣不下 氧化应激 血清金属离子 抗氧化指标 |
| DOI： |
| 分类号: |
| 基金项目:山东农业大学青年博士基金项目 |
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| Study on the comparison between the serum levels of some ions in dairy cows with and without RFM |
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| Abstract: |
| 【Objective】 The research analyzed the correlation between the level of trace element ion and oxidative stress in dairy cows for studying the mechanism of retention of fetal placenta (RFM).【Method】 The blood samples were collected in cows without (n=5)and with RFM (n=5) at parturition and 12h after parturition.The serum levels of eight element ions (K+、Na+、Ca2+、Mg2+、Cu2+、Fe2+、Zn2+ and Mn2+) at parturition and 12h after parturition were measured by atomic absorption spectrophotomrtry and the anti-oxidative indexes in the serum of the cows with and without RFM at parturition were determined by 722 visible spectrophotometry.【Result】 The result indicated that the concentrations of Mn2+、Zn2+ and Cu2+（P<0.01）,Ca2+ and Mg2+（P<0.05）in dairy cows with RFM decreased significantly compared with those in the serum in normal cows, but the concentration of Fe2+ was significantly higher than that in the serum in normal cows（P<0.01）.The activities of Zn-SOD,Cu-SOD and total SOD and GSH-Px in cows with RFM were significantly lower than those in normal cows（P<0.01）but the levels of MDA and OH· in cows with RFM were significantly higher than those in normal cows （P＜0.05）.【Conclusion】 It was concluded that there existed correlation between concentrations of several trace elements and oxidative stress in dairy cows with RFM. |
| Key words: dairy cow RFM oxidative stress mineral ion antioxidative index |
