| 摘要: |
| [目的]揭示西瓜核雄性不育发生的分子机理.[方法]应用mRNA差异显示技术,研究西瓜核雄性不育材料Se18不育株和可育株雄花花蕾中基因表达的差异.[结果]获得了西瓜细胞核雄性不育两用系不育相关特异cDNA片段T12C/B0315S-359和育性相关特异cDNA片段T12C/B0315F-175、T12G/B0318F-260.经过序列比较,T12C/B0315S-359与美洲杨树一段未知的cDNA序列同源性较高(82%);T12C/B0315F-175与葫芦科植物中编码细胞色素C装配蛋白的ycf5基因高度同源(98%);T12G/B0318F-260与植物中硫氧还蛋白过氧化物酶(Thioredoxin peroxidase,TPx)基因高度同源(84%).[结论]经序列分析初步认为,这3个片段与西瓜细胞核雄性不育的发生有重要相关性. |
| 关键词: 西瓜 雄性不育 基因分析 差异显示 |
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| 基金项目:国家“863”高技术研究与发展计划项目（2001AA241174） |
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| Isolation of the cDNA fragment of watermelon genic male sterility related genes using DDRT-PCR |
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| Abstract: |
| 【Objective】 The research was done in order to study the molecular mechanism of genic male sterility in watermelon.【Method】 mRNA differential display was used to analyze the differential expression of genes between male sterile flower buds and male fertile flower buds in male sterile watermelon Se18.【Result】 Through identification of repeated tests,the differential fragments T12C/B0315S-359 were obtained in male fertile flower buds;two differential fragments were obtained in male sterile flower buds:T12C/B0315F-175 and T12G/B0318F-260.The T12C/B0315S-359 fragment was 82% homologous with a code sequence of an unknown protein which involved in vascular development of quaking aspen.The result of GenBank BLAST showed that the T12C/B0315F-175 fragment had 98% nucleotide similarity to the ycf5 gene encoding cytochrome C assembly protein in cucurbitaceous plants.The homology research indicated that T12G/B0318F-260 fragment was of 84% of homology with code sequence of mRNA of thioredoxin peroxidase in plants.【Conclusion】 After sequence research,the three differential fragments were related to genic male sterility in watermelon Se18. |
| Key words: watermelon male sterility genetic analysis DDRT |
