| 摘要: |
| [目的]了解引起奶牛乳房炎的最主要病原菌葡萄球菌的耐药性,为奶牛乳房炎的防治提供参考依据.[方法]于2006～2007年在泰安地区5个规模化奶牛场,采集128份临床乳房炎奶牛乳样,用传统的细菌分离、培养和生化鉴定方法,对其中的葡萄球菌进行了分离,用K-B纸片扩散法对葡萄球菌的耐药性进行了检测调查.[结果]本次调查中共分离到29株葡萄球菌,其中金黄色葡萄球菌14株(14/29,47.4%),产色葡萄球菌3株(3/29,10.3%),木糖葡萄球菌、腐生葡萄球菌、松鼠葡萄球菌、表皮葡萄球菌和人葡萄球菌各2株(2/29,6.9%),头葡萄球菌和模仿葡萄球菌各1株(1/29,3.3%);葡萄球菌对头孢拉定和阿米卡星高度敏感,对临床上常用的林可霉素、红霉素、四环素、复合磺胺均存在不同程度的耐药性.金黄色葡萄球菌对万古霉素的平均抑菌圈直径为19.1 mm,凝固酶阴性葡萄球菌对万古霉素的平均抑菌圈直径为20.3 mm.[结论]金黄色葡萄球菌在分离葡萄球菌中仍占主导地位,万古霉素对葡萄球菌具有较强的体外抗菌活性,未发现耐药株. |
| 关键词: 奶牛 乳房炎 葡萄球菌 药敏试验 万古霉素 |
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| Isolation, identification, medicine sensitivity test of Staphylococcus of dairy cow mastitis |
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| Abstract: |
| 【Objective】 Mastitis is mainly caused by Staphylococcus. One hundred and twenty eight milk samples were collected from 5 cattle farms in Taian from 2006 to 2007. 【Method】 Traditional plate culture method and biochemistry experimentation were used to isolate and identify Staphylococcus. Antibiotic Susceptibility Test was evaluated by K-B paper dispersion method.【Result】 29 Staphylococcus strains were isolated from these samples, including S.aureus 14/29（47.4％），S.chromogenes 3/29 (10.3％)，S.xylosus、S.cpidermidis、S.siaproghyticus and S.sciuri 2/29（6.9％）respectively,S.caplitis and S.simulans 1/29（3.3％）respectively.The results indicated that all Staphylococcus was hypersensitive to cephradine and Amikacin and partially resistant to Clindamycin,Teracycline,Trythromycin and TMP/SMZ which are used frequently in clinic.Average diameter of circle that controlled S.aureus and Coagulase-negative Staphylococcus for Vancomycin was 19.1 mm and 20.3 mm respectively. 【Conclusion】S.aureus was primarily pathogenic bacteria of Staphylococcus.Staphylococcus which could resist Vancomycin was not found. |
| Key words: cow mastitis Staphylococcus medicine sensitivity test Vancomycin |
