| 摘要: |
| [目的]扩增了牛RXRG基因的序列,并对其进行了生物信息学分析.[方法]提取牛肌肉组织总RNA,利用RT-PCR技术,对牛RXRG基因进行了克隆,将得到的2条片段分别重组到PMD19-T载体并进行了序列测定;利用DNAMAN软件拼接得到2条片段序列,并运用DNAMAN软件及在线工具对拼接所得到的序列进行了生物信息学分析.以牛肾脏、肝脏、睾丸、肺、脾脏、瘤胃、子宫、小肠、心脏、卵巢和肌肉等11 个组织为材料,对牛RXRG基因在组织中的表达进行了分析.[结果]获得了长度为1 811 bp的牛RXRG基因cDNA,其由10个外显子组成,编码463个氨基酸;该基因与人、猪、猕猴、小鼠、大鼠在氨基酸序列上分别有89.5%,93.4%,87.2%,83.9%和85.2%的同源性.[结论]获得了牛RXRG基因长度为1 811 bp的cDNA 序列,牛RXRG基因除在肌肉组织中高度表达外,在其他组织中均不表达. |
| 关键词: 牛 RXRG RT-PCR 生物信息学 组织表达谱分析 |
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| 基金项目:国家“十一五”科技支撑计划重大项目“农林动植物育种工程”（2006BAD01A10）；国家“十一五”高技术研究发展计划项目（“863”计划项目）(2006AA10Z197) |
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| cDNA cloning and biological information analysis of cattle RXRG gene |
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| Abstract: |
| 【Objective】 Tow cDNA sequences of the RXRG gene were obtained and analyzed in biological information.【Method】 The total RNA was extracted from cattle muscle tissue.This experiment cloned cattle RXRG gene cDNA by RT-PCR,and the products were cloned in PMD19-T vector and sequenced.These two sequences were connected by DNAMAN software,and the connected sequence was analyzed by the DNAMAN software and online tool in biological information.The mRNA content was tested in kidney,liver,testicle,lung,spleen,rumen,uterus,small intestine,heart,and ovary and muscle tissues of cattle.【Result】 A 1 811 bp long cDNA sequence was obtained.The analysis of amino acid sequence indicated that cattle RXRG gene consisted of 10 exons and coded 463 amino acids.Homologous comparison with some animals indicated that cattleRXRG cDNA shared 89.5％,93.4％,87.2％,83.9％,85.2％ similarity in nucleic acid sequence with human,pig,macaca,mouse and rattus.【Conclusion】 A 1 811 bp long cDNA sequence of RXRGgene was cloned.RXRG cDNA was found in muscle tissue by the analysis of its expression in various tissues,but not found in other tissues. |
| Key words: bovine RXRG RT-PCR bio-informatics tissue expression analysis |
