| 摘要: |
| [目的]4CL是木质素生物合成途径中的关键合成酶,通过对4CL基因的遗传进化分析,为其研究和利用提供理论依据.[方法]利用生物信息学手段,对4CL基因完整cDNA和编码氨基酸序列进行数据挖掘,利用Mega软件、ExPASy的Prosite数据库、NCBI的Conserved Domains数据库,进行4CL基因核酸和蛋白质水平上的遗传进化分析.[结果]构建了4CL基因的系统发生树,揭示出植物中的4CL基因聚类与植物分类大体一致,主要以基因家族的形式存在,但基因家族中部分基因存在着结构和功能的分化,4CL基因中存在着GC含量偏高和偏低两大类基因.4CL基因编码的氨基酸序列保守区高度相似,但是在单、双子叶两大类植物之间,其氨基酸平均含量存在者较大差异.[结论]在植物的进化过程中,虽然4CL基因较保守,但部分4CL基因发生了分化,体现在基因的结构和功能方面,在4CL基因的研究中必须充分考虑到这种分化. |
| 关键词: 木质素 4CL基因 遗传进化分析 |
| DOI： |
| 分类号: |
| 基金项目:四川省应用基础研究基金项目(05JY029-101)；四川省教育厅资助项目(2003031022)；四川省青年基金项目(041100)；西南科技大学重点项目(2006031030) |
|
| Genetic and evolutionary analysis of lignin biosynthase 4CL gene |
|
|
| Abstract: |
| 【Objective】 4CL (4-coumarate:CoA ligase) is an important biosynthase in the lignin biosynthesis.Through genetic and evolutionary analysis of 4CL gene,some theoretic foundation could be found for researches of 4CL gene.【Method】 With the bioinformational tools,the complete sequences of 4CL cDNA and corresponding amino acid have been found from the gene database.With the Mega,Prosite database of ExPASy,Conserved Domains database of NCBI,the genetic and evolutionary analysis was respectively carried out in nucleotides and proteins level.【Result】 The Phylogenetic tree of 4CL genes was constructed.The sorting of 4CL genes tree and the sorting of plants were mainly identical.There were 4CL family genes in the 4CL genes,in which the divergence of the construction and function were found.Two kinds of 4CL genes,which respectively contain high percents and low percents of GC contents,were found. The conversed amino acid blocks of individual 4CL gene were highly similar.But there were differences in the average amino acid contents between the monocots and dicots. 【Conclusion】 In the process of plants evolution,some 4CL genes diverged,although most of 4CL genes were reserved.The divergence of structure and the function of 4CL gene were included.The divergence should be fully considered in the 4CL gene research. |
| Key words: lignin 4CL gene genetic and evolutionary analysis |
