| 摘要: |
| [目的]研究拟南芥FT(Flowering Locus T)基因在拟南芥植物开花过程中的重要作用及其mRNA的长距离运移能力.[方法]将FT基因功能序列及其突变序列mFT(起始密码子突变为终止密码子)连接入马铃薯X病毒(Potato Virus X,PVX)载体和去除外壳蛋白(Coat Protein,CP)序列的芜菁缩叶病毒(Turnip crinkle virustruncated coat protein,TCV△CP)载体中,经体外反转录得到病毒目的片段RNA,然后分别用PVX-FT侵染烟草,TCV△CP-FT侵染拟南芥.[结果]人工接种PVx-FT可以诱导烟草开花;TCV△CP-FT和TCV△CP-mFT人工侵染拟南芥叶片后,经RT-PCR检测发现,在侵染的叶片中与其上部新生叶片中有TCV△CP-FT和TCV△CP-mFT序列,表明FT mRNA序列可以帮助失去细胞间移动能力的TCV△CP恢复移动能力,并且在顶芽中检测出明显条带,经测序证实为FT基因序列.[结论]FT可以诱导短日照品种烟草在长日照条件下开花,而且FT mRNA功能序列可以协助因失去CP序列而没有细胞间移动能力的TCV△CP恢复移动能力. |
| 关键词: FT(Flowering Locus T)基因 马铃薯X病毒载体（PVX） 芜菁缩叶病毒（TCV） RT-PCR |
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| 基金项目:国家留学基金委中英卓越联合培养博士生计划资助项目 |
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| Research on moving ability and function of Arabidopsis Flowering Locus T |
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| Abstract: |
| 【Objective】 The paper studied the function of Arabidopsis FT (Flowering Locus T),which plays a key role in floral pathway,and FT mRNA trificking ability in Arabidopsis.【Method】 In this research FT sequence was cloned and sequence mFT (FT start codon replaced by stop codon) was mutanted into PVX(Potato Virus X) and TCV△CP (Turnip crinkle virus vector truncated coat protein sequence),PVX-FT and TCV△CP-FT RNA were transcripted in vitro,inoculated on tobacco and on Arabidopsis respectively.【Result】 PVX-FT can trigger tobacco flowering.The sequence of TCV△CP-FT were found and TCV△CP-mFT were found in the new leaves on top of Arabidopsis by using RT-PCR checking and sequence testing,which means FT mRNA sequence can help TCV△CP sequence that lost the moving ability to move from inoculated leaves to new young leaves.【Conclusion】 All these results show FT can trigger short-day tobacco flowering in long-day,and FT mRNA can help heterogeneous viral sequence TCV△CP recover cell-to-cell long distance moving ability. |
| Key words: FT (Flowering Locus T) PVX TCV RT-PCR |
