摘要: |
[目的]探讨黄芪和板蓝根对猪细小病毒(PPV)的体外抑制作用.[方法]选用中草药黄芪、板蓝根作为抗病毒药物,利用细胞病变(CPE)抑制试验,测定黄芪与板蓝根单独使用及等体积合用,在PK-15单层细胞上对猪细小病毒的抑制作用.[结果]板蓝根单独使用时,体外试验对PPV有明显的抑制作用,对PPV的最小直接杀灭质量浓度为0.63 mg/mL,最小阻断质量浓度为0.31 mg/mL黄芪单独使用时对PPV无明显的抑制作用;板蓝根与黄芪等体积合用时,对PPV的抑制作用显著增强,最小直接杀灭质量浓度提高为0.31 mg/mL,最小阻断质量浓度提高为0.038 mg/mL.[结论]黄芪与板蓝根联合使用抗病毒能力增强,且毒性较弱. |
关键词: 黄芪 板蓝根 猪细小病毒(PPV) |
DOI: |
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基金项目:河南省科技攻关项目(322011000) |
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Study on inhibitive effect of Radix Astragali and Radix Isatidis in vitro on porcine parvovirus |
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Abstract: |
【Objective】 The study was to investigate inhibitive effect on porcine parvovirus by Radix Astragali and Radix Isatidis in vitro.【Method】 Chinese herbs Radix Astragali and Radix Isatidis were adopted as antiviral drugs by utilizing the cytopthic effect(CPE) inhibitive experiment to measure the inhibitive effect on porcine parvovirus(PPV) by pig kidney(PK)-15 cell monolayer,when Radix Astragali and Radix Isatidis were used alone and association by equal volume. 【Result】 Radix Isatidis has obviously inhibitive effect on experiment in vitro by individual using,the minimum concentration of direct kill was 0.63 mg/mL,the minimum concentration of blocking was 0.31 mg/mL;the Radix Astragali has not obviously inhibitive effection in experiment in vitro used alone.When Radix Astragali and Radix Isatidis were associated by one to one,the inhibitive effect on PPV has been enhanced obviously;the minimum concentration of direct killing was 0.31 mg/mL;and the minimum concentration of blocking was 0.038 mg/mL. 【Conclusion】 The anti-virus capability were enhanced when Radix Astragali and Radix Isatidis were used in combination, at the same time toxicity were weakened. |
Key words: Radix Astragali Radix Isatidis porcine parvovirus(PPV) |