| 摘要: |
| [目的]分离筛选木质纤维素材料的高效降解菌,为植物农药残渣的资源化利用及其他废弃有机物的合理利用提供依据.[方法]采用纤维素刚果红平板培养基,对采自陕西秦岭山区的土样和腐烂枝干进行初筛,再以川楝残渣为惟一碳源进行摇瓶培养复筛,最后对获得的高纤维素酶活力菌株进行形态学和分子生物学鉴定.[结果]初筛获得了38株能够产生纤维素酶的菌株.在此基础上,对其中15株菌进行液体发酵并测定纤维素酶活性,得到4株纤维索酶活性较高菌株F4-1、F1-3、F10-2和F2-3.经形态观察和18S rDNA基因片段分析,将这4株菌分别归为枝状枝孢菌(Cladosporium cladosporioides)、萨氏曲霉菌(Aspergillus sydowi)、瓦克青霉菌(Penicillium waksmanii)和小不整球壳属(Plectosphaerella).[结论]获得的4株真菌均具有较高的降解天然纤维素的能力. |
| 关键词: 纤维素酶 菌株筛选 形态学特征 分子鉴定 |
| DOI： |
| 分类号: |
| 基金项目:国家西部开发重大项目(2004BA901A14)；陕西省“13115”项目（2007ZDGC-04） |
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| Screening and identification of superior fungus degraded cellulose |
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| Abstract: |
| 【Objective】 This study isolated superior cellulose decomposing strains to provide rational application of plant-pesticide residue and other waste organic materials.【Method】 The fungus were firstly screened in Congo red plate for cellulase,and then cultured in residues of chinaberry as only carbohydrate supply.The fungus were identified by morphological and molecular technology.【Result】 The 38 strains of microbes producing cellulose were isolated by some distinct zones of clearing in cellulose-congo red argar medium.By measuring the enzyme activity of liquid culture filtrates,four strains F4-1,F1-3,F10-2 and F2-3 with high cellulose activity were obtained.These were confirmed as Cladosporium cladosporioides,Aspergillus sydowi,Penicillium waksmanii and Plectosphaerella sp.by the biological property and sequence of 18S rDNA.【Conclusion】 These strains can degrade natural cellulose specially. |
| Key words: cellulase screening of strain morphological characteristic molecular identification |
