| 摘要: |
| [目的]揭示水分对玄参生长前期地上部分生长的影响,探讨玄参抗逆反应中各保护酶的作用,为玄参规范化栽培中的水分供应提供理论依据.[方法] 在严重干旱(土壤含水量为400～450 g/kg,SD)、中度干旱(土壤含水量为500～550 g/kg,MD)、适宜水分(土壤含水量为700～750 g/kg,CK)和过湿水分(土壤含水量为800～850 g/kg,HW)处理条件下,对玄参株高、叶面积、叶片叶绿素含量、可溶性蛋白含量及4种保护酶(超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX))的活性进行测定,研究水分对玄参株高、叶面积、叶片叶绿素含量、可溶性蛋白含量以及4种保护酶活性的影响.[结果] 与适宜水分相比,严重干旱、中度干旱和过湿水分处理均明显抑制了玄参株高和叶面积的增长(P<0.05);处理第13～33天玄参叶片叶绿素含量偏低.各处理对叶片可溶性蛋白含量的影响表现为13 d时促进可溶性蛋白的合成,其后则对可溶性蛋白的合成表现出抑制作用.SOD和APX活性在处理过程中变化比较复杂,而POD和CAT活性在处理前期较高,后期逐渐下降.[结论] 不适宜的水分供应可抑制玄参株高和叶面积的增加;造成玄参叶片叶绿素含量在处理的第13～33 d间偏低;导致玄参叶片可溶性蛋白合成在13 d时增加,13天后则受到抑制.水分处理对SOD和APX活性影响较大,对POD和CAT活性的影响仅在发生在处理前期;严重干旱和中度干旱处理对玄参各保护酶活性具有较大影响. |
| 关键词: 玄参 生长发育 土壤水分 保护酶活性 |
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| 基金项目:教育部新世纪优秀人才支持计划项目(NCET-04-0954)；西北农林科技大学拔尖人才支持计划项目(05-22) |
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| Effects of soil water content on the growth of the overground parts and antioxidant capability in leaves of Scrophularia ningpocnsis Hemsl |
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| Abstract: |
| 【Objective】 The study was done in order to learn the relationship of prophase growth and the dynamic variations of SOD,POD,CAT and APX activity of Scrophularia ningpocnsis Hemsl.and water condition.【Method】 The shoot height,leaf area,chlorophyll content,soluble protein content and dynamic variations of SOD,POD,CAT and APX activity in leaves were studied under the condition that Scrophularia ningpocnsis Hemsl.were treated with four levels of soil water content which were respectively serious drought(θf =400-450 g/kg,SD),moderate drought (θf =500-550 g/kg,MD),check sample (θf =700-750 g/kg,CK) and high water (θf800-850 g/kg,HW) in order to learn the effect of different water treatments on the shoot height,leaf area,chlorophyll content,soluble protein content and dynamic variations of SOD,POD,CAT and APX activity in leaves.【Result】 The research showed that the shoot height and single leaf area of Scrophularia ningpocnsis Hemsl.were significantly restrained under SD,MD and HW treatment(P<0.05),and the chlorophyll contents in leaves were also lower than that of the CK treatment.The effect of SD,MD and HW treatment on the soluble protein content was more significant than the CK treatment on the 13th day of the treatment,and were restrained after the 13th day.SOD and APX activity changed in a complicated way during the treatment;POD and CAT activity decreased with the prolonging of the treatment time.【Conclusion】 Under SD,MD and HW treatment,the growing of shoot height and single leaf area were restrained;The chlorophyll contents of SD,MD and HW treatment were a little lower than that of CK treatment between the 13th and the 33th day afer treatment;Soluble protein increased in the 13th day, and was restrained afer the 13th day under SD,MD and HW treatment.The effect of water treatment was more significant for SOD and APX activity,and the effect for POD and CAT activity happened in the early stage of the treatment.The enzymes were more sensitive in the SD treatment and the MD treatment. |
| Key words: Scrophularia ningpocnsis Hemsl. growth water condition activity of the protective emzyme |
