| 摘要: |
| [目的]探讨槐花水溶性多糖提取的工艺条件及其抗氧化活性.[方法] 采用四因素三水平正交试验设计,研究了提取温度、时间、次数和料液比等因素对槐花多糖提取率的影响,考察了Sevage法、三氯乙酸法、盐酸法、胰蛋白酶 Sevage法对槐花多糖所含蛋白的脱除效果,并对槐花多糖的还原能力,及清除羟基自由基、超氧阴离子自由基能力进行了研究.[结果] 提取槐花多糖的工艺条件为:料液比1∶25,提取温度80 ℃,提取时间6 h,提取次数4次.槐花多糖的最佳清除蛋白方法为胰蛋白酶Sevage法,蛋白清除率为85.23 %.抗氧化试验结果表明,槐花多糖具有较好的抗氧化活性,是一种效果好、安全性高的新型天然抗氧化物质.[结论] 获得了槐花水溶性多糖提取的最佳工艺条件,该工艺下槐花多糖的得率为3.40%. |
| 关键词: 槐花多糖 提取工艺 抗氧化活性 |
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| 基金项目:陕西省自然科学基金项目（2006B18） |
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| Studies on extraction and antioxidant function of polysaccharides form Sophora japonica |
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| Abstract: |
| 【Objective】 The extraction technology and antioxidant of polysaccharides from Sophora japonica were studied.【Method】 The effect of each factor such as temperature,time,times and ratio of solid-liquid on the extraction of the water-soluble polysaccharides from Sophora japonica was investigated using the methods of single factor test and orthogonal design test.Sevage method,TCA method,HCL method and combining enzyme and Sevage method were used to remove protein of polysaccharide from Sophora japonica.Antioxidant activities of polysaccharides from Sophora japonica were evaluated by reduction capabilities, scavenging effects to hydroxyl radicals and oxygen radicals.【Result】 The result showed that the highest extraction rate of polysaccharides from Sophora japonica could be obtained with a ratio of solid to liquid as 1∶25,80 ℃ for 6 h and 4 times of extraction.The enzymatic+Sevage removal of protein was an effective method and the removal of protein reached 85.23%.Anti-oxidative results showed polysaccharides from Sophora japonica had the effect of anti-oxidation.Sophora japonica polysaccharides were a potential product of natural,safe and effective antioxidant.【Conclusion】 The best condition for the extraction of polysaccharides from Sophora japonica was obtained with the yield of extraction 3.40%。 |
| Key words: Sophora japonica polysaccharides extraction technology antioxidant activity |
