| 摘要: |
| [目的]研究波尔山羊级进杂交后代GH基因第4、5外显子的遗传多态性及其与体重性状的相关性。[方法] 采集波尔山羊及其与关中奶山羊级进杂交F1、F2和F3代羊的血样,利用PCR-SSCP技术分析其GH基因第4、5外显子的多态性,并用最小二乘法研究了第5外显子多态性与体重性状的关系。[结果] 第4外显子在山羊群体中不存在多态性,而第5外显子在该群体存在多态性(有AA,AB,AC,AD 4种基因型)；第5外显子发生了2处突变,AB基因型在237 bp位发生了C→T突变,AD基因型在第289 bp位发生了C→A突变,但突变均没有导致氨基酸的变化。波尔山羊及其F1代群体中AD型个体的初生重极显著高于AC型(P>0。01)；在F2代群体中,AB型个体的初生重和1月龄体重显著高于AA型(P<0。05)；不同基因型个体的3月龄体重均差异不显著(P>0。05)。[结论] 第4外显子在山羊群体中不存在多态性,而第5外显子在该群体存在多态性。GH基因第5外显子可能对波尔山羊级进杂交后代体重性状有影响。 |
| 关键词: GH基因 PCR-SSCP 遗传多态性 山羊 |
| DOI： |
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| 基金项目:国家高技术研究发展计划（863）项目（2007AA10Z167）；国家“十一五”奶业重大科技支撑项目（2006BAD04A11） |
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| Analysis of polymorphism and weight traits of the 4th and 5th exon of GH gene in descendants by grading hybridization from goats |
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| Abstract: |
| 【Objective】 The experiment studied the relation between the genetic polymorphisms and the weight traits of the fourth and fifth exon of GH gene of Boer and their progeny. 【Method】 Blood samples were collected from boar goat and their F1,F2 and F3 generation.PCR-SSCP and sequencing were applied to analyze the effect of the genetic variation,and least square was applied to analyze the correlation of different genotypes and body weight in fifth exon of GH gene.【Result】 There were no polymorphisms in the fourth exon and four genotypes of AA,AB,AC,AD in the fifth exon.Two polymorphisms were detected in the fifth exon AB genotype at 237 site(C→T) and AD genotype at 289 site(C→A),and two mutations occurred in the fifth exon.The association of the variation in the fifth exon of GH gene with the birth weight trait in the population were analyzed.The results showed that the birth weight of the individuals with a genotype AD was significantly higher(P<0.01) than that with a genotype AC in the Boer and their F1 generation.But in the F2 generation the individuals with a genotype AB had a higher birth weight and one month weight than that with a genotype AA (P<0.05).【Conclusion】 The result showed that there were no polymorphisms in the fourth exon and the polymorphism in the fifth exon of gGH gene had significant effect on weight traits. |
| Key words: growth hormone gene PCR-SSCP genetic polymorphism goat |
