| 摘要: |
| [目的]建立高效稳定的菊叶薯蓣组织培养快繁体系,以保持品种的遗传稳定性.[方法]分别以室内和田间培育的墨西哥菊叶薯蓣藤茎茎段为外植体起始材料,比较依次用0.55%次氯酸钠、体积分数70%酒精和1.0g/L HgCl2消毒不同时间对外植体的消毒效果,并通过不同植物生长调节剂及其浓度组合筛选适合菊叶薯蓣组培快繁的茎段不顶芽诱导培养基、继代培养基和生根培养基.[结果]以室内培养的墨西哥菊叶薯蓣藤茎为起始材料,依次用0.55%次氯酸钠消毒5 min,体积分数70%酒精消毒1 min,1.0 g/L HgCl2消毒8 min,最后用无菌水清洗3次,获得材料的污染率可控制在5%;筛选出茎段不定芽诱导培养基为MS+3.0 mg/L BA+0.2 mg/L NAA,不定芽诱导数可达4.3个,诱导率达95%,且不定芽质量较好;将分化的不定芽转至MS+1.0 mg/L BA+0.2 mg/L IBA继代培养基,单茎芽增殖率可达到3.5;茎芽在1/2 MS+1.0 mg/L IBA生根培养基上可以诱导出良好的根系.[结论]按上述方法培育的菊叶薯蓣生根试管苗炼苗后,移栽至珍珠岩+粗沙+田园土按体积比2:1:1配置的介质中,移栽成活率可以达到85%以上,表明所建立的墨西哥菊叶薯蓣组培快繁体系是可行的. |
| 关键词: 菊叶薯蓣 组织培养 茎段 |
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| 基金项目:国家林业局“948”引进项目（2002-30） |
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| Adventitious shoot regeneration from nodal segments of Dioscorea composite |
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| Abstract: |
| 【Objective】 A rapid micropropagation method of Dioscorea composite was constructed through tissue culture to keep the stability of heredity.【Method】 Room-cultured and field-cultured tender vines were selected as start materials.Different time and concentration's combinations among 0.55% sodium hypochlorite (V/V),70% ethanol,1.0 g/L HgCl2 were carried out.The different type and concentration of plant growth regulators was also tried,then the regeneration medium,elongation medium,propagation medium were determined.【Result】 The tender vines from the plants cultivated in room was suitable for explants establishment,the percentage of contamination was under 5% using the sterile method(0.55% sodium hypochlorite(V/V) 5 min,volume fraction of 70% ethanol 1 min, 1.0 g/L HgCl2 8 min,three steps were carried out in consequence).The best induction medium was the MS basal medium supplemented with 3.0 mg/L BA and 0.2 mg/L NAA,the highest shoot induction rate reached 4.3 shoots per explant,the shoot inducted by BA was of good quality in height and status.The adventitious shoots can be propagated on the MS basal medium supplemented with 1.0 mg/L BA and 0.2 mg/L IBA,then roots can be induced on the 1/2MS basal medium,containing 1.0 mg/L IBA.All of these above presented showed that the protocol can be used as a good method for Dioscorea composite rapid multiplication in large scale.【Conclusion】 Using the above method,rooted plantlets were transferred to plastic pots containing medium composed of autoclaved pearlite-coarse sand-soil (2/1/1),the survival rate was over 85%.The research showed that the protocol developed for in vitro regeneration of nodal segments of Dioscorea composite was feasible. |
| Key words: Dioscorea composita tissue culture nodal segment |
