| 摘要: |
| [目的]建立鸡传染性支气管炎病毒(IBV)快速、准确的检测方法.[方法]根据GenBank中已经发表的793/B型和多株IBV的N基因和S1基因,对比分析后以N基因的保守序列设计合成了1对引物,跨度为582bp;以793/B的S1基因设计合成了1对特异型引物,跨度为891 bp;建立了能检测出IBV并能够同时鉴定出793/B血清型的实验室一次性PCR诊断方法.[结果]多重RT-PCR检测表明,793/B血清型可出现582和891 bp 2条核酸片段,而其他血清型只出现582 bp 1条核酸片段,新城疫病毒、传染性喉气管炎、鹅副粘病毒则无任何条带出现.[结论]所建立的TR-PCR方法具有一定的特异性,可用于IBV的快速检测和793/B血清型的临床诊断. |
| 关键词: 多重RT-PCR 传染性支气管炎 793/B血清型 检测方法 |
| DOI： |
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| 基金项目:济南市科技攻关项目（41054） |
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| Detection of infectious bronchitis virus and differentiation of the 793/B serotype by multiplex RT-PCR |
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| Abstract: |
| 【Objective】 A Multiplex RT-PCR was optimized to simultaneously detect IBV and 793/B to save detection time.【Method】 Two sets of primers were designed according to the sequences of 793/B and other IBV at the Genbank.【Result】 The products of 582 and 891 bp were generated only with RNA from 793/B,where as there was only 582 bp product with RNA from other IBV.However,the RT-PCR failed to detect NDV,ILTV and GPV.【Conclusion】 The results showed that the established multiplex RT-PCR technique provided a more sensitive method for diagnosis and epizootic study of the IBV. |
| Key words: multiplex RT-PCR infectious bronchitis virus 793/B strain detection method |
