| 摘要: |
| [目的]对天女木兰种子进行不同浸种和催芽处理试验,探讨快速解除种子休眠的机理和促进种子萌发的技术.[方法]将天女木兰种子分别用100,200,300 mg/L GA3和清水浸种48 h,然后在室内进行低温层积((4±1)℃、150 d)和人工控制变温层积((4±1)℃、50 d→(17 1)℃、25 d→(4±1)℃、50 d→(17±1)℃、25 d)催芽处理,并在层积过程中定期取样测定天女木兰种子的胚长占种子长的比例、呼吸速率、可溶性糖含量、可溶性蛋白含量和蛋白酶活性.[结果]用GA3浸种后进行变温层积催芽的天女木兰种子,胚发育优于相应的低温层积催芽,并随着GA3质量浓度的提高催芽效果越好,且温度条件起主导作用;2种层积催芽方法下,天女木兰种子的呼吸速率均提高,且经GA3浸种的种子呼吸速率与清水浸种的种子相差不大;天女木兰种子中可溶性糖和可溶性蛋白含量在2种层积催芽方法下均比层积前有不同程度提高,不同GA3质量浓度浸种处理间差异不大;用2种层积催芽方法催芽的天女木兰种子蛋白酶活性升高,并且随着GA3质量浓度的提高而增强,层积后期蛋白酶活性低于层积初期,变温层积处理的蛋白酶活性高于低温层积处理.[结论]经300 mg/L GA3浸种并进行变温层积的天女木兰种子催芽效果最佳,温度条件在天女木兰种子的层积催芽过程中起主导作用. |
| 关键词: 天女木兰 催芽试验 层积 GA3 |
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| 基金项目:沈阳农业大学博士后基金资助项目（777-230409） |
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| Effect of different soaking and accelerating germination disposals on forced germination of M.sieboldii k.koch seeds |
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| Abstract: |
| 【Objective】M.sieboldii k.koch’seed was researched by different soaking and accelerating germination disposals in order to discuss the mechanism of relieveing fastly dormancy and technique of facilitating germination.【Method】M.sieboldii k.koch’seeds were soaked in the liquor of GA3 100,200,300 mg/L and water for 48 h,then stratified them in temperature of indoor stratification（（4±1）℃,150 d） and temperature of artificial controlling（（4±1）℃,50 d→（17±1）℃,25 d→（4±1）℃,50 d→（17±1）℃,25 d）.In the process of stratification samples were fetched termly and embryo measured to kernel length，respiratory rate，solube sugar，solube protein and protease.【Result】 The results showed the growth of the embryo of cold warm stratification of GA3 treatments was better than cold stratification of GA3 treatments,and in the increasing of consistency of GA3,the effect of forced germination was more distinct,and the main function was the temperature;After stratification,the respiratory rate enhanced,but the difference of the GA3 and water soaked seeds was little;solube sugar and solube protein was higher than before in different degrees;After the two different stratifications,the protease increased,and the protease enhanced in the increasing of the consistency of GA3 ,in the late period of the stratification the protease was lower than the early stratification, the protease of cold warm stratification was higher than cold stratification.【Conclusion】 Effect of M.sieboldii k.koch’seed,soaking by GA3 300 mg/L and alternating temperature was best,tempreture had dominant role in the process of forced germination of M.sieboldii k.koch seeds. |
| Key words: M.sieboldii K．Koch’seed germination accelerating test stratification GA3 |
