| 摘要: |
| [目的]建立雷公藤愈伤组织诱导培养体系,为雷公藤通过组织培养生产次生代谢产物奠定基础.[方法]以雷公藤枝条扦插苗的根、幼茎、叶为外植体,研究基本培养基、激素、外植体类型对愈伤组织诱导的影响,并检测了不同外植体愈伤组织乙酸乙酯提取物的杀虫活性.[结果]MS培养基有利于雷公藤愈伤组织的诱导;雷公藤的根、茎、叶3种外植体均可用来诱导愈伤组织;供试激素中,2,4-D的诱导效果较NAA好,KT的效果优于6-BA,低质量浓度的KT与2,4-D组合能促进愈伤组织的诱导和生长;根、茎、叶3种愈伤组织提取物对粘虫幼虫均有明显的拒食活性,对粘虫幼虫的生长发育有明显的抑制作用,48 h拒食中质量浓度(AFC50)分别为27.8,53.1和76.1mg/mL.[结论]雷公藤愈伤组织诱导的最佳培养基及激素组合为:MS 1.0 mg/L 2,4-D 0.5～1.0 mg/L KT;根愈伤组织提取物对粘虫幼虫的拒食活性最强. |
| 关键词: 雷公藤 组织培养 愈伤组织诱导 粘虫 拒食活性 |
| DOI： |
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| 基金项目:国家“十五”科技攻关项目（2004BA516A04） |
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| Study on callus induction and insecticidal activities of Tripterygium wilfordii Hook.f. |
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| Abstract: |
| 【Objective】 The study established Tripterygium wilfordii callus induction training system and laid a foundation for the production of tissue culture secondary metabolites.【Method】 The root,stem and leaves of T.wilfordii branch cuttings as explants were used to study the effects of the basic media，hormones,explant type on their calluses induction and the insecticidal activity was detected through different explants callus ethyl acetate extract.【Result】 MS medium was suitable for the callus induction of T.wilfordii.The roots,stems and leaves explants of T.wilfordii could be used to induce callus.2,4-D was better than NAA.KT was better than 6-BA.KT of low concentrations and 2,4-D combination could promote callus induction and growth.Antifeedant activity of different callus extracts on Mythimna separata larvae was evident.Effect of different callus extracts on the growth and development of M.separate larvae was inhibited significantly.48 h AFC50 were 27.8，53.1，76.1 mg/mL.【Conclusion】 MS+1.0 mg/L 2,4-D + 0.5-1.0 mg/L KT was best for callus induction of T.wilfordii.Root callus extract showed the strongest antifeedant activity on M.separata larvae. |
| Key words: Tripterygium wilfordii Hook.f. tissue culture callus induction Mythimna separata Walker antifeedant activity |
