| 摘要: |
| [目的]建立葡萄离体培养快繁体系,探索葡萄组培苗的脱毒技术,以防治葡萄卷叶病的发生.[方法]依据葡萄茎尖分化、伸长和生根3阶段中所需激素比例的不同,以1/2 MS、B5和GS为基础培养基,并适当调节激素之间的比例,筛选适合不同时期的最佳培养基;同时研究了葡萄微茎尖大小、热处理方式和时间、不同病毒钝化剂处理等对葡萄组培苗的脱毒效果.[结果]筛选出从葡萄茎尖分化到生根的最佳激素配比,其培养时间比常规微茎尖培养缩短12～19 d;在脱毒处理中,将热处理与茎尖培养相结合,使成活率和脱毒率较单纯茎尖培养提高7.5%和8.5%,其中变温比恒温处理成活率提高15.6%,而脱毒率相差不大;病毒钝化剂处理与微茎尖相结合培养结果表明,病毒钝化剂对葡萄病毒具有良好的抑制作用,但对茎尖的生长发育有影响.[结论]葡萄微茎尖培养与热处理及病毒钝化剂处理相结合,是葡萄组培苗较好的脱毒方法. |
| 关键词: 葡萄卷叶病毒 微茎尖培养 热处理 病毒钝化剂 脱毒技术 |
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| 基金项目:宁夏回族自治区自然科学基金项目（nz0619）；宁夏大学自然科学基金项目 |
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| Study on techniques of elimination of Grapevine roll-leaf virtus |
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| Abstract: |
| 【Objective】 Building system of grape tissue culture for speeding propagation and rearching technology of virus-free grape test-tub seedlings are the most effective protection measures for grapevine leaf-roll virus (GLRaV). 【Method】 From stem tip differentiation to stem elongate and radication,tissue culture plant needed hormone in various proportions.Through adjusting hormone in various proportions as 1/2 MS,B5 and GS basic culture media,the optimum culture media in three stages were selected.The virus-free elimination effect in tissue culture of stem tip,heat treatment model and time and different virus passivator treatment were also studied.【Result】 At three stages,a series of culture media with the optimum hormone proportions was prepared,which could shorten the culture time by 12-19 days as compared with the media used commonly.Cooperation between heat treatment and tissue virus-free culture of stem tip,the survival rate of the seedling and GLRaV elimination rate increased 7.5% and 8.5% respectively,compared with singlar tissue Virus-free culture of stem tip.Moreover,in the survival rate,the changable heat treatment increased 15.6% while the GLRaV effect in grapevine showed little difference.The addition of virus passivor in media can inhibit propagation of virus.It,however,had negative effect on the survival rate of seedling.【Conclusion】 It was better virus-free method combining the tissue virus-free culture of stem tip with heat treatment and virus passivaor treatment. |
| Key words: Grapevine leaf-roll virus (GLRaV) tissue culture of stem tip heat treatment virus passivation elimination of virus |
