| 摘要: |
| [目的]M853-2是一个通过杂交和回交选育的普通小麦一柔软滨麦草易位系,苗期对中国小麦生产上流行的条锈菌(Puccinia striiforms f.sp.tritici)主要生理小种表现良好抗性.研究易位系M853-2抗条锈菌的遗传规律,对揭示其遗传机制和抗源的筛选具有重要意义.[方法]以感病品种铭贤169和易位系M853-2作亲本,通过杂交制备F2代种子,用人工接种方法研究M853-2及其杂交后代对小麦条锈菌不同生理小种的苗期抗性,并进行了遗传分析,最后对其中一个接种群体进行了SSR标记.[结果]M853-2对条中29的抗锈性遗传受2对显性和1对隐性基因的独立控制,对条中30的抗锈性遗传受2对隐性和1对显性核基因以及3对隐性胞质基因的共同作用,对条中31的抗锈性遗传受2对显性(互补作用)基因的独立控制,对Su-4的抗锈性遗传受1对显性和1对隐性核基因以及2对显性(互补作用)胞质基因的共同控制,对Su-11的抗锈性遗传受1对显性基因的独立控制,将该抗锈基因暂命名为YrElm2,并对该接种群体利用BSA法进行了SSR标记.从305对SSR引物中筛选到1个位于4BL上的SSR标记Xgwm495,连锁分析表明,YrElm2与Xgwm495的遗传距离为7.60 cM,该抗病基因位于4BL上.[结论]普通小麦-柔软滨麦草易位系M853-2对小麦条锈病有良好的抗性,对所接种的菌系CY29、CY31和Su-11表现为核基因遗传,对CY30和Su-4表现为与核质互作有关的抗病性遗传,说明易位系M853-2可以作为抗源在我国小麦抗锈育种中应用. |
| 关键词: 普通小麦 柔软滨麦草 抗条锈病基因 遗传分析 SSR标记 |
| DOI： |
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| 基金项目:教育部长江学者和创新团队发展计划项目（No.200558）；陕西省科技攻关项目（2005K02-G1-1） |
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| Genetic analysis and microsatellite makers of a resistance gene YrElm2 in wheat from Elymus mollis (Trin.) Hara |
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| Abstract: |
| 【Objective】 Crossing triticum aessivum 7182 with Elymus mollis (Trin.)Hara,a translocation line to be attained and named M853-2 shows excellent resistance to the dominant races of Puccinia striiforms f.sp.tritici prevalent in China at seedlings.Inheritance of stripe rust resistance gene in M853-2 is important for revealing the resistance to stripe rust and as a resistant resource.【Method】 F2 seeds were prepared by crossing Mingxian169,a susceptible variety and M853-2.Resistance of M853-2 and its progenies was analyzed genetically by artificial inoculation with different races of the pathogen at seedlings.【Result】 The resistance to the race CY29 in M853-2 was controlled by two dominant and one recessive gene independently,and the resistance to the race CY30 was controlled by two dominant and one recessive genes (they are karyplasms genes) and three recessive cytoplasmic genes,and the resistance gene to CY31was controlled by two dominant complement genes,and the resistance to Su-4 was controlled by one dominant and one recessive karyplasms gene and two dominant cytoplasmic genes,while the resistance to the race Su-11 was controlled by one dominant gene named YrElm2.To develop molecular markers for YrElm2,bulk segregation analysis combined with 305 SSR markers were used,one SSR marker,Xgwm495 on the 4BL was associated with YrElm2.The genetic distance between Xgwm495 and YrElm2 was about 7.60 cM.YrElm2 was located on the 4BL.【Conclusion】 The results indicated that the M853-2 as a resistance resource had a considerable value in wheat breeding for rust resistance. |
| Key words: commom wheat Elymus mollis (Trin.)Hara resistance gene genetic analysis SSR marker |
