| 摘要: |
| [目的]探讨了Aroclor 1254对小鼠2-细胞和8-细胞胚胎体外发育的影响.[方法]在体外培养的小鼠2-细胞和8-细胞胚胎培养液中,分别添加质量浓度为0.05,0.25,1.25和6.25 μg/mL的Aroclor 1254,并设Aroclor 1254溶剂(乙醇)对照组和空白对照组,比较不同质量浓度的Aroclor 1254对小鼠2-细胞和8-细胞胚胎体外发育的影响.[结果]0.05 μg/mL Aroclor 1254影响2-细胞胚胎的孵化(57.7%),与溶剂对照组(66.7%)差异显著(P＜0.05);0.25 μg/mL Aroclor 1254组,2-细胞胚胎的囊胚发育率(72.5%)及囊胚孵化率(23.2%)与溶剂对照组(84.2%,66.7%)差异极显著(P＜0.01);1.25 μg/mL Aroclor 1254组,2-细胞胚胎发育到8-细胞阶段的比率(32.1%)极显著低于溶剂对照组(P＜0.01);6.25 μg/mL Aroclor 1254组,2-细胞胚胎发育到4-细胞和8-细胞阶段的比率(58.1%和29.6%)极显著低于溶剂对照组(P＜0.01).0.05 μg/mL Aroclor 1254对8-细胞胚胎发育无明显影响;但当质量浓度提高到0.25 μg/mL时,Arolor 1254显著影响8-细胞胚胎的孵化(64.7%,P＜0.05);1.25 μg/mLAroclor 1254组,8-细胞胚胎囊胚发育率(68.6%)及孵化率(25.5%)极显著低于溶剂对照组(92.4%,75.7%)(P＜0.01);6.25 μg/mL Aroclor 1254组8-细胞胚胎不能继续发育.[结论]Aroclor 1254对小鼠胚胎体外发育存在明显的剂量-毒性关系,随着培养液中Aroelor 1254质量浓度的增加,对小鼠早期胚胎的发育毒性表现越早;胚龄越小,毒性敏感性越强. |
| 关键词: Aroclor 1254 毒性 胚胎 体外发育 小鼠 |
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| 基金项目:陕西省自然科学基金项目（99SM66） |
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| Effect of Aroclor 1254 on early mouse embryo development in vitro |
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| Abstract: |
| 【Objective】 The aim of this study was to investigate the effect of Aroclor 1254 on mouse embryo(2-cell and 8-cell stage) development in vitro.【Method】 Aroclor 1254 of 0.05,0.25,1.25 and 6.25 μg/mL were added to the mouse embryo(2-cell and 8-cell stage) culture media respectively,solvent(ethanol) control group and blank control group were set and then the effects of Aroclor 1254 with different concentration to the mouse embryo developmental competence cultured in vitro were compared.【Result】 The results indicated that Aroclor 1254 with 0.05 μg/mL could significantly depress the hatching rate(57.7%) of the 2-cell stage embryos compared with the solvent control group(66.7%)(P<0.05);The blastular ratio(72.5%)and hatching rate(23.2%) were degraded significantly at 0.25 μg/mL of Aroclor 1254 compared with that of corresponding solvent control group(84.2%,66.7%,respectively)(P<0.01);When the concentration of Aroclor 1254 elevated to 1.25 μg/mL,the embryo developmental proportion from 2-cell to 8-cell stage(32.1%) was notably cut down compared with that of the dissolvent control group.And the ratio of 2-cell stage embryos development to the 4-cell and 8-cell stage(58.1% and 29.6% respectively) were significantly lower than that of dissolvent control group(P<0.01),after the 2-cell stage embryos were exposed to Arcolor 1254 of 6.25 μg/mL.Aroclor 1254 at 0.05 μg/mL had no significant impact on the developmental competence of 8-cell embryos,but when the concentration was elevated to 0.25 μg/mL,the hatching rate(64.7%) of the 8-cell embryos was significantly decreased,compared with that of the solvent control group (P<0.05).The blastocyst developmental rate(68.6%) and hatching rate(25.5%) of the 8-cell embryos with 0.25 μg/mL of Aroclor 1254 were obviously lower than that of the corresponding solvent control group(92.4%,75.7%,respectively)(P<0.01).No embryo can develop continously when the concentration of Aroclor 1254 was evaluated to 6.25 μg/mL.【Conclusion】 The conclusion was that Aroclor 1254 depressed the mouse embryo development according to the dose-toxicity in vitro;as the dose of Aroclor 1254 increased,the toxical effect on mouse embryo development would appear earlier and act more fiercely.The younger the embryo was,the more sensitive it was to Aroclor 1254. |
| Key words: Aroclor 1254 toxicity embryo in vitro mouse |
