| 摘要: |
| [目的]通过多基因聚合创制小麦抗条锈病新种质,并筛选小麦抗条中32号小种基因的RAPD标记.[方法]用分别抗条中32号、31号小种和水源14号小种的花育888-5、西农1376和212 3个育种材料进行杂交、复交,并花培纯合,对来自3个抗条锈育种材料的不同抗性基因进行聚合,建立DH系.用115条随机引物对该DH群体中抗条中32号小种基因进行RAPD标记分析.[结果]通过基因聚合,获得抗条中31号和32号小种的材料6个,占总材料的7.32%;抗条中31号和水源14号小种的材料6个,占总材料的7.32%;抗条中32号和水源14号小种的材料1个,占总材料的1.22%;未获得对3个供试小种均有抗性的DH材料.引物S20扩增出670 bp的多态性片段,该特异条带重复性强,S20可作为小麦抗条中32号小种基因的特异标记.[结论]创制了聚合2个抗条锈流行小种基因的抗条锈新种质13个.与抗条中32号小种基因紧密连锁的特异RAPD标记为S20. |
| 关键词: 条锈病 生理小种 基因聚合 花药培养 分子标记 |
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| 基金项目:国家“863”高新技术研究与发展计划项目（2003A207100）；陕西省重大科技攻关项目（2006KZ06-G1） |
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| Pyramiding of resistance genes to stripe rust in wheat and molecular markers of resistance gene to CY32 |
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| Abstract: |
| 【Objective】 New wheat germplasm with stripe rust resistance was developed by several anti-disease gene pyramiding of the wheat materials and RAPD technique was used in resistance gene molecular marker of Tiaozhong No.32 race in wheat.【Method】 Huayu 888-5,Xinong 1376 and Xinong 212 of No.32,No31 and Shui 14 were crossed,multipli-crossed and then DH of the 3 rust resistance materials was established.115 primers were used to marker gene molecular RAPD to No.32 race.【Result】 The results showed that 6 doubled hyploid materials were both resistant to CY31,CY32 with percentage 7.32%,5 double hyploid materials were both resistant to CY31,Shui14 with percentage 6.10%,1 doubled hyploid materials were both resistant to CY32,Shui14 with percentage 1.22%.There were not doubled hypluid materials with resistant to CY32,CY31,Shui14.DH materials of resistance to physiological race CY32 were analysed with 210 primers by RAPD technique,1 additional DNA fragments were amplified repeat and steadily in resistant parent and resistance gene pool by RAPD technique,that is the fragments amplified respectively by the primer S20.But no rust resistance DH material to 3 testers was obtained.The additional DNA fragment amplified by the primer S20 was about 670bp,this additional DNA fragments were amplified repeat edly by individuals.【Conclusion】 13 wheat new germplasms with stripe rust resistance were developed by anti-disease gene pyramiding and S20 molecular markers linked to resistance gene to CY32 were selected by RAPD technique. |
| Key words: stripe rust physiological race gene pyramiding anther culture molecular marker |
