| 摘要: |
| [目的]从白僵菌代谢物中分离对天牛有毒性的蛋白,并测定其N-末端氨基酸序列.[方法]从球孢白僵菌培养滤液中分离蛋白类代谢物,以重要林业害虫松墨天牛幼虫为试虫筛选毒蛋白,对筛选的毒蛋白进行PAGE电泳后转至PVDF膜上,测定其末端氨基酸序列.[结果]从白僵菌代谢物中共分离出5种毒蛋白,其中毒性最强的是PrBb4蛋白,其对松墨天牛幼虫的致死率为56.67%.PrBb4蛋白N-末端起始的5个氨基酸序列为谷氨酸(Glu)→脯氨酸(Pro)→丝氨酸(Ser)→异亮氨酸(Ile)→缬氨酸(Val).[结论]白僵菌代谢物中含杀天牛毒蛋白PrBb4,其N-末端氨基酸序列为Glu→Pro→Ser→Ile→Val. |
| 关键词: 白僵菌 代谢物 毒蛋白 氨基酸序列 松墨天牛 |
| DOI： |
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| 基金项目:国家重点基础研究发展规划（973）项目（G19990160） |
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| Isolation and ammonia acid N-terminal sequence of the toxic protein to Longhorn Beetle |
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| Abstract: |
| 【Objective】 The toxic proteins to longhorn beetle were isolated from Beauveria bassiana culture filtration,and N-terminal ammonia acid sequence was measured.【Method】Toxic proteins were screened by bioassay with forest pest Monochamus alternatus larvae as the targets.N-terminal ammonia acids sequence of the protein PrBb4 was measured after PrBb4 was transferred on the PVDF.【Result】 Five toxic proteins were isolated from B.bassiana culture filtration.It shows that the toxicity of PrBb4 is the highest in all proteins,whose mortality of PrBb4 is 56.67%.Five ammonia acids sequence of N-terminal end from the beginning is Glu→Pro→Ser→Ile→Val. 【Conclusion】 There is toxic protein PrBb4 in the B.bassiana culture filtration,the first ammonia acid of N-terminal end is Glu. |
| Key words: Beauveria bassiana secretion toxic protein ammonia acid sequence monochamus alternatus |
