| 摘要: |
| [目的]探究3种不同类型辣椒细胞质雄性不育材料的不育分子机理,寻找与雄性不育相关的基因.[方法]采用高盐-蛋白酶K法提取辣椒细胞质雄性不育系及其保持系的绿色叶片线粒体DNA(mtDNA),根据已报道的辣椒细胞质雄性不育相关基因orf456序列设计特异引物,利用PCR反应,从3种不同类型辣椒细胞质雄性不育材料线粒体基因组中均扩增出330 bp左右大小的条带.[结果]经克隆测序及序列分析表明,3种不同类型辣椒细胞质雄性不育材料所获片段序列完全一致,大小为330 bp,命名为CMS330,与orf456核酸序列同源性达99%.[结论]3种不同类型辣椒细胞质雄性不育材料具有相似的不育分子机理. |
| 关键词: 辣椒 细胞质雄性不育 线粒体DNA orf456 |
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| 基金项目:国家自然科学基金项目(30771467,30571262)；“十一五”国家科技支撑计划项目(2006BAD01A7);教育部春晖计划项目（Z2005-2-71005） |
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| Cloning and sequence analysis of the specific mitochondrial DNA fragment related to cytoplasmic male sterility in pepper |
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| Abstract: |
| 【Objective】 Three different types of cytoplasmic male sterile mechanism in pepper were investigated to find out the DNA fragment related to cytoplasmic male sterility.【Method】 Pepper mitochondrial DNA (mt DNA) was extracted from green leaves of cytoplasmic male sterile line and its maintainer line based on high salt-proteinase K method.The specific fragments at around 330 bp were amplified by PCR using special primers designed on the basic of pepper cytoplasmic male sterility associated gene orf456 in three kinds of cytoplasmic male sterile lines.【Result】 Further sequences analysis showed that these three fragments are identical and termed CMS330 of 99% homology with orf456.【Conclusion】 It reflected that they have the similar male sterile mechanism. |
| Key words: pepper cytoplasmic male sterility mitochondrial DNA (mt DNA) orf456 |
