| 摘要: |
| [目的]为葡萄抗白粉病育种的辅助选择提供理论依据.[方法]以抗病的中国野生葡萄白河-35-1与感病的欧洲葡萄佳利酿杂交亲本及其F1、F2代为试材,通过对520个随机引物的筛选,从佳利酿中获得了葡萄感白粉病基因的RAPD标记OPV06-1100,并在欧洲葡萄、中国野生华东葡萄和美洲野生葡萄中进行了分析验证.[结果]经克隆、测序,RAPD标记OPV06-1100实际长度为1 016 bp.该标记与欧洲葡萄黄酮醇合成酶基因有92%的同源性,与13条欧洲葡萄叶片非生物胁迫数据库的EST序列有89%～97%的同源性;与3条来自欧洲葡萄感染葡萄皮尔斯病原菌后转录反应获得的EST序列有93%～95%的同源性;与拟南芥感白粉病基因PMR6序列有27.1%的同源性.[结论]OPV06-1100为葡萄属植物感白粉病基因的RAPD标记.该标记为认识葡萄感病基因和基因组以及标记辅助育种奠定基础. |
| 关键词: 葡萄 白粉病 感病基因 RAPD标记 序列分析 |
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| 基金项目:国家自然科学基金项目（30170653） |
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| RAPD marker and its sequence analysis of susceptible gene to Uncinula necator in Vitis |
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| Abstract: |
| 【Objective】 The study is to provide theoretical basis for selecting Vitis resistance Uncinula nector in breeding.【Method】 RAPD analysis was carried out by using the parents and their F1 and F2 individuals of interspecific hybridization combination Baihe-35-1×Carignane (Resistant×Susceptible).A total 520 random primers were screened on the DNA of resistant and susceptible materials.OPV06-1100,a RAPD marker of susceptible gene to Uncinula necator in Vitis was obtained from V.vinifera cultivar Carignane,and was tested among V.vinifera cultivars,V.pseudoreticulata of Chinese wild Vitis species and clones,American wild Vitis species and clones (varieties) and Parthenocissus Planch. 【Result】 The length of the RAPD marker is 1 016 bp by cloning and sequencing.Additionally,the sequence of OPV06-1016 was analysed.OPV06-1100 shows 92% identity with V.vinifera FLS5 gene for flavonol synthase,shows 89% to 97% identity with 13 EST sequence abiotic stressed leaves of V.vinifera,shows 93% to 95% identity with 3 EST sequence from V.vinifera infected with Xylella fastidiosa,shows 27.1% identity with gene PMR6 from Arabidopsis thaliana of the susceptibility to Uncinula necator.【Conclusion】 OPV06-1100 is a PAPD marker linked to Uncinula necator susceptible genes in Vitis.The marker will provide a foundation to know susceptible genes and genomic of grape as well as marker-assisted breeding. |
| Key words: Vitis Uncinula necator susceptible gene RAPD marker sequence analysis |
