| 摘要: |
| [目的]研究鸡传染性支气管炎病毒(IBV)澳大利亚T株S1基因全序列.[方法]采用RT-PCR技术,对IBV T株S1基因进行扩增、克隆和测序.[结果]测序结果表明,IBV T株S1基因大小为1 739 bp.与GenBank中的29株IBV S1基因进行比较发现,IBV T株与吉林疫苗株(JAAS)S1基因核苷酸同源性为99.8%,有4个核苷酸的差异,其中有3个碱基发生非同义突变,氨基酸同源性为99.4%;与其余28株IBV S1基因核苷酸同源性为73.8%～84.4%,氨基酸同源性为66.8%～85.9%.进化分析发现T株与JAAS株之间的亲缘关系很近,与其他IBV株的亲缘关系均较远.[结论]JAAS株是T株的变异病毒,IBV T株的变异病毒在我国存在. |
| 关键词: 传染性支气管炎病毒 澳大利亚T株 S1基因 序列分析 |
| DOI： |
| 分类号: |
| 基金项目:国家“十五”食品安全重大攻关专项（2001BA804A30-11） |
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| Cloning and sequence analysis of S1 gene of Australian T strain of infectious bronchitis virus |
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| Abstract: |
| 【Objective】 The research is to study S1 glycoprotein gene nucleotide sequence of infectious bronchitis virus（IBV）Australian T strain.【Method】 S1 glycoprotein gene of IBV-T strain was amplified by reverse transcription-polymerase chain reaction(RT-PCR),then was cloned and sequenced.【Result】 The sequencing result showed that the sequence consisted of 1 739 bp.When the nucleotide sequence of S1 gene of T strain was compared with that of 29 strains of IBV in GenBank,homology between T strain and JAAS strain was 99.8% at nucleotide level,only four nucleotide differences (A498G，C974T，T1 251A，G1 396T) were found,leading to three amino acids substitution (F325S,Q417H,S466A),and the deduced amino acid homology was 99.4%.However,nucleotide homologies with other strains ranged between 73.8% to 84.4% and the deduced amino acid homologies ranged between 66.8%~85.9%.Evolution analysis indicated that the T strain was closely related to JAAS,very colse to the other strains of IBV.【Conclusion】 The result showed that JAAS strain was a variant virus of T strain and there was a variant virus of IBV-T strain in our country. |
| Key words: infectious bronchitis virus Australian T strain S1 gene sequence analysis |
