| 摘要: |
| [目的]确定蛋白酶水解核桃蛋白的最佳工艺条件.[方法]在单因素分析的基础上,采用响应面分析方法对核桃蛋白的水解条件进行了优化.[结果]胰蛋白酶水解核桃蛋白的最佳反应条件为:反应时间4.0 h,底物质量浓度27 g/L,温度53.4 ℃,pH8.0,加酶量(E/S) 8.3 g/kg,各因素对核桃蛋白水解的影响顺序为温度＞加酶量＞底物质量浓度.[结论]实际验证表明,利用优化的水解条件可取得较好的水解效果,水解度可达14.497%. |
| 关键词: 核桃蛋白 胰蛋白酶 水解条件 工艺优化 |
| DOI： |
| 分类号:S433.4 S482.39 |
| 基金项目:科技部科技富民强县项目(国科发农字（2006315)） |
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| Optimization Study on hydrolysis of walnut protein by trypsin protease |
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SONG Wei TANG Guang-hui FENG Jun-tao ZHANG Tao WANG Ya-lu GUO Hai-peng ZHANG Xing
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| Abstract: |
| 【Objective】 The study is to obtain the optimal condition of hydrolysis for walnut protein by using protease.【Method】 The response surface methodology was used to evaluate the factors affecting trypsin protease hydrolysis based on single factor test.【Result】 The optimum reaction conditions about hydrolysis of walnut protein by trypsin protease were reaction time 4.0 h,substrate concentration 27 g/L.reaction temperature 53.4 ℃,pH8.0 and enzyme/substrate 8.3 g/kg.The sequence of factors that worked on the hydrolysis of walnut protein were as below:temperature>enzyme>substrate concentration.【Conclusion】 The result of applying this feasible extracting condition to manufacturing process is satisfactory,with the degree of hydrolysis 14.497%. |
| Key words: walnut protein trypsin protease hydrolyzing conditions technological optimization |
