| 摘要: |
| [目的]对放线菌A19菌株发酵液中的次生代谢产物进行初步研究,以了解其抑菌活性及其稳定性.[方法]采用抑制菌丝生长法、管碟法、盆栽试验等方法,测定了A19菌株发酵液的抑菌活性和发酵液中有效成分的热稳定性及酸碱稳定性;并以活性追踪为指导,采用大孔吸附树脂、硅胶柱层析、高效液相色谱等技术对其有效成分进行了分离.[结果]室内生测发现,A19菌株发酵液对10种供试病原真菌均有明显的抑制作用,其中对小麦根腐病菌的抑制效果最好,抑制率达100%;对枯草牙孢杆菌、金黄色葡萄球菌和L-链球菌3种病原细菌也有较好的抑制作用,其中对枯草芽孢杆菌的抑制作用最强,抑菌圈直径达到21 mm.盆栽试验表明,A19菌株发酵液对小麦白粉病有一定的保护作用和治疗作用,防治效果分别为54.3%和65.0%.A19菌株发酵液对热稳定,对酸碱不稳定.从A19菌株的发酵液中分离得到15-1,15-2和15-3 3种化合物,其中15-2鉴定为7-羟基-3异丁基六氢吡咯[1,2-α]吡嗪-1,4-二酮.[结论]放线菌A19菌株发酵液抗菌谱较广,具有较好的研究价值. |
| 关键词: A19菌株 次生代谢产物 发酵产物 生物活性 |
| DOI： |
| 分类号:S661.106.2 |
| 基金项目:国家重点基础研究发展计划项目（2003CB114404） |
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| Primary studies on the secondary metabolic products of strain A19 of actinomycetes |
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FAN Hong-zhu TONG Yan-an LU Shi-hua
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| Abstract: |
| 【Objective】 Primary studies on the secondary metabolic products of an actinomycete strain,A19 were conducted to know its fungicidal activity and stability.【Method】 Fungicidal activity of the fermentation broth of strain A19 was studied by means of inhibition of mycelium growth rate,agar diffusion method and pot tests respectively.And its stability to temperature,acid and alkali were tested.The bioactive compounds in the fermentation broth were isolated by the methods of macroporous adsorption resin,silica gel column chromatography and high performance liquid chromatography.【Result】 The results of in vitro test showed that the fermentation broth appeared obvious inhibition against ten tested pathogens,and the best inhibition was observed in the treatment of Bipolaris sorokiniana.It could also control effectively against three tested bacteria,in which the treatment of Bacillus subtilis had the best efficacy.The results of pot test indicated that the fermentation products had both protection and therapeutic efficacy on wheat powdery mildew.Compound 15-1,15-2 and 15-3 isolated from the fermentation products,15-2 was identified as 7-hydroxy-3-isobutylhexahydro pyrrolo［1,2-α］pyrazine-1,4-dione.【Conclusion】 The antagonistic spectrum of the fermentation broth of strain A19 was extensive and worth to study. |
| Key words: strain A19 secondary metabolic products fermentation products bioactivity |
