| 摘要: |
| [目的]克隆牛BMPRⅠA基因cDNA全长,以进行生物信息学分析和组织表达谱研究.[方法]运用生物信息学方法,结合RT-PCR和SMART RACE技术,对牛BMPRⅠA基因进行cDNA全长克隆和生物信息学分析,并通过RT-PCR方法进行了组织表达谱研究.[结果]克隆得到了牛BMPRⅠA基因4 067 bp的cDNA全长序列,通过核酸序列分析发现,该基因编码532个氨基酸,与人、黑猩猩、小鼠、大鼠、犬和红原鸡在核酸序列上分别有91%,93%,89%,88%,91%和82%的同源性;在氨基酸序列上分别有97%,95%,96%,95%,97%和91%的同源性.通过生物信息学分析发现,牛BMPRⅠA蛋白包含一个信号肽序列和一个跨膜区序列,其成熟蛋白可能位于细胞膜上.在牛卵巢、肝、肌肉、小肠、脂肪、子宫、肾脏、心肌、肺、胰腺、睾丸、乳腺、瘤胃、脾脏、淋巴、胸腺等组织中都检测到有BMPRⅠA基因表达.[结论]牛BMPRⅠA基因是一个功能重要、进化保守的基因,具有广泛的组织表达谱. |
| 关键词: 牛 BMPRⅠA基因 cDNA克隆 组织表达谱 |
| DOI： |
| 分类号:S852.659.5 Q786 |
| 基金项目:国家农业科技跨越计划（2004跨20） |
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| Cloning and tissue expression analysis of cDNA encoding the cattle BMPRⅠA gene |
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SHANG Shu-wen HOU Ji-bo XU Gong-bao NIU Ming-fu WANG Xiu-qing HE Jia-hui CHEN Pu-yan JIANG Ping
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| Abstract: |
| 【Objective】 The study was to clone and analyze the full-length cDNA sequence of the cattle BMPRⅠA gene and the expression in 12 tissues.【Method】 The BMPRⅠA gene full-long cDNA was cloned by RT-PCR,and SMART RACE and analyzed by bioinformatics ways.【Result】 The expressions were tested in 12 tissues by RT-PCR.The cattle BMPRⅠA gene full-long cDNA is 4 067 bp,which encodes 532 amino acids and shares 91%，93％，89％，88％，91％ and 82％ similarity in nucleic acid sequences and 97％，95％，96％，95％，97％ and 91％ similarity in amino acid sequences with human,chimpanzee,mouse,rat,dog and red jungle fowl,respectively.The cattle BMPRⅠA protein has a signal peptide and a transmembrane domain.The mature protein is located in cell membrance by informatics analysis.The BMPRⅠA gene are expressed in ovary,liver,skeleton muscle,small intestine,fat,uterus,kidney,heart,lung,pancreas,testis,galactophore,rumen,spleen,lymph and thymus gland.【Conclusion】 The BMPRⅠA is an important and conservative gene,which is broadly expressed in all kinds of tissues. |
| Key words: cattle BMPRⅠA cDNA clone tissue expression analysis |
