| 摘要: |
| [目的]建立对卵清蛋白(OVA)免疫耐受的Babl/c小鼠模型.[方法]分别设立A、B、C、D、E、F试验组及相应对照组.A、B组小鼠口服OVA,隔天1次,共服5次,口服剂量分别为50或100 μg/只;C、D、E、F组小鼠经尾静脉注射OVA 1次,剂量分别为5,10,50和100 μg/只;分别于口服完成7 d后或尾静脉注射5 d后免疫小鼠,免疫后15 d,MTT法测定小鼠OVA特异性淋巴细胞增殖情况,间接ELISA测定小鼠血清抗OVA的IgG水平.C、D组小鼠分别进行二、三次免疫并检测抗体.[结果]A、B组小鼠淋巴细胞增殖的刺激指数(Simulate index,SI)分别为0.603±0.03和0.715士0.08,增殖效果不佳;C、D组小鼠SI值分别为0.096±0.01和0.083±0.01,淋巴细胞不增殖;E、F组小鼠淋巴细胞增殖效果与正常小鼠无差异.第一次免疫后,各组小鼠OVA抗体效价与相应对照组小鼠的OVA抗体无显著差异;C、D组在二免、三免后抗体水平持续下降,不呈现再次应答规律.[结论]小鼠口服小剂量OVA可以建立不完全细胞免疫耐受,经尾静脉注射小剂量OVA可以建立完全免疫耐受. |
| 关键词: 卵清蛋白 免疫耐受 淋转试验 ELISA |
| DOI： |
| 分类号:F301.2 |
| 基金项目:国家重点基础研究发展计划(973计划)
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教育部长江学者和创新团队发展计划
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教育部科技计划
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国家自然科学基金 |
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| Induction and identification of peripheral tolerance to ovalbumin administered intravenously or orally |
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QU Zhi-peng WANG Xiao-jie XU Liang-sheng JIA Tao KANG Zhen-sheng
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| Abstract: |
| 【Objective】 The study is to establish a model tolerance to ovalbumin.【Mathod】 Mice gastrically intubated ovalbumin (OVA) had been divided into experiment group A,B,C,D,E and F and their corresponding control groups.Group A and B were at the dose of 50 or 100 μg every other day for 5 times.The rest were i.v.at the dose of 5,10,50 or 100 μg once for each.The oral administration groups and the intravenously injected groups were challenged with 200 μg OVA,at 7 days or 5 days interval,respectively.15 days later,mice were sacrificed. Lymphocytes responses were assessed by MTT method and antibody were tested by ELISA.And then group C and D injected 5 or 10 μg were exposed to the second and the third immunization.Antibody was detected at 15 days interval for 4 times.【Result】 The regimen of OVA oral administration group A and B can induce Ag-specific lymphocytes profound suppression,with simulate index 0.603±0.03 and 0.715±0.08,repectively.And the anti-OVA IgG level was remarkablly affected.As to the intravenous group C and D,these lymphocytes from mice i.v.5 or 10 μg OVA were totally unresponsiveness in all mice tested,with index 0.096±0.01 and 0.083±0.01,respectively while group E and F were normally responded.Antigen-specific B cells were normally responded with the first immunization.But they had no reactivity to repetitious immunization and anti-OVA IgG were gently declined in 2 months.【Conclusion】 Low dose of OVA intravenous injection can initiate specific immune tolerance while the oral administration in this experiment can just suppress the responses of T cells and B cells evidently. |
| Key words: ovalbumin immune tolerance lymphocyte proliferate assay ELIS |
