| 摘要: |
| 为了探讨大枣多糖的超声波辅助提取最佳工艺条件及其单糖组成，研究了超声波频率、液料比、提取时间和提取温度对大枣多糖提取率的影响，以正交试验优化了超声波辅助提取的工艺条件；并采用1-苯基-3-甲基-5-吡唑啉酮（PMP）进行柱前衍生，反向高效液相色谱法（HPLC）紫外检测了单糖组分。结果表明，超声辅助提取大枣多糖的最佳工艺条件为：超声波频率28 kHz、料液比1 g∶10 mL、提取时间2.5 h、提取温度70 ℃，在此条件下提取率可达7.51％；提取的大枣多糖包括L-鼠李糖、D-果糖、葡萄糖、D-半乳糖、L-阿拉伯糖等5种单糖，D-甘露糖未检出。提示本试验确定的大枣多糖最佳工艺条件可行；PMP柱前衍生HPLC分析实现了糖类物质的良好分离，可作为大枣多糖结构分析的新方法。 |
| 关键词: 大枣多糖 超声波提取 PMP 高效液相色谱 |
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| Ultrasonic-assisted extraction of jujube polysaccharide and analysis in HPLC after derivatization |
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| Abstract: |
| In order to research the optimal ultrasonic-assisted extraction condition of jujube polysaccharide and its monosaccharide composition,the paper studies four factors of the jujube polysaccharide yield,including the ultrasonic frequency，proportion of raw material and water,extraction time and extraction temperature.Through orthogonal test,the best extraction condition is determined.After derivatization of saccharides by PMP，the monosaccharide composition of jujube polysaccharide is analyzed by HPLC using UV detection.The result shows the best extraction condition is 28 kHz for ultrasonic frequency,1 g∶10 mL for proportion of raw material and water,2.5 h for extraction time,70 ℃ for extraction temperature,under which polysaccharide yield can reach 7.51％.Its monosaccharide composition consists of L-rhamnose,D-fructose,glucose,D-galactose,and L-arabia,while D-mannose is not detected.So the extraction condition of jujube polysaccharide is feasible;derivatization of saccharides by PMP can segregate saccharides very well,so it can be a new method to analyze the texture of jujube polysaccharide. |
| Key words: jujube polysaccharide ultrasonic-assisted extraction PMP HPLC |
