| 摘要: |
| 为了利用生物信息学方法筛选绵羊微卫星标记,利用SSRIT和SSRFinder软件对绵羊UniGene数据库的4 081个簇进行搜索,筛选绵羊EST-SSRs标记。结果表明,从绵羊UniGene数据库中搜索到微卫星136个,含有微卫星的序列121个,占整个EST序列数据库的3.0%,其中双碱基重复47个,三碱基重复54个,四碱基重复3个,五碱基重复4个,六碱基重复28个。在这些微卫星序列中,AC/TG重复在双碱基类型中最丰富,CTG重复在三碱基类型中最常见,分别占双碱基和三碱基微卫星序列总数的64%和29%。根据筛选到的微卫星序列设计并合成引物30对,在设计的30对引物中,20对引物有扩增产物,且条带清晰,其中有2对引物在小尾寒羊品种内呈现多态。 |
| 关键词: 绵羊 表达序列分析 微卫星；SSR |
| DOI： |
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| 基金项目:国家高技术研究发展计划(2006AA10Z199国家“863”计划项目(2006AA10Z199);农林动植物育种工程项目（2006BAD01A11）);农林动植物育种工程项目 |
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| Analysis of microsatellite markers from sheep UniGene |
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| Abstract: |
| In this study,SSRs markers were developed by bioinpormcotics methods and a total number of 4 081 sheep unigene sequences were downloaded.These UniGene sequences were screened for the presence of perfect microsatellites by using SSRIT and SSRFinder soft tools.A total number of 136 SSRs were identified from 121 EST sequences.The frequency of EST containing SSR is 3.0%.The trinucleotide repeat motif is the most abundant SSR,accounting for 39.71%,followed by 34.56% for dinucleotide repeats.Among the dinucleotide repeats,AC/TG was the most abundant repeat motif,accounting for 64% of all dinucleotide repeats.While CTG was the most abundant type in the trinucleotide repeat types,accounting for 29%.30 primer pairs were designed from microsatellite and 20 pairs could show clear PCR products by electrophoresis for further polymorphism analysis.The result in this paper provided a basis for the development and further application of EST-SSR markers in sheep. |
| Key words: sheep expressed sequence tags microsatellite simple sequence repeat |
