| 摘要: |
| 以克新12号、克新13号、东农303和早大白4种马铃薯试管苗茎段为材料,研究各品种的组织培养体系。结果发现,克新13号和克新12号的最适愈伤组织诱导培养基为MS+30 g/L蔗糖+8 g/L琼脂+2 mg/L 6-BA+1 mg/L 2,4-D;东农303为MS+30 g/L蔗糖+8 g/L琼脂+2 mg/L 6-BA +0.5 mg/L 2,4-D;早大白为MS+30 g/L蔗糖+8 g/L琼脂+2 mg/L 6-BA+0.5 mg/L 2,4-D。用上述最适诱导培养基培养,各品种的愈伤诱导率分别为100%,100%,100%和90%。克新13号愈伤组织分化的最佳培养基为MS+30 g/L蔗糖+8 g/L琼脂+4.0mg/L 6-BA+0.1 mg/L NAA+1.0 mg/L GA3;克新12号和早大白为MS+30 g/L蔗糖 +8 g/L琼脂+2.0 mg/L 6-BA+0.5 mg/L NAA+1.0 mg/L GA3;东农303为MS +30 g/L蔗糖+8 g/L琼脂 +2.0 mg/L 6-BA+0.1 mg/LNAA+1.5 mg/L GA3。用上述最适诱导培养基培养,各品种的分化率分别为80%,90%,100%,76.7%。 |
| 关键词: 马铃薯 克新12号 克新13号 东农303 早大白 愈伤诱导 愈伤分化 |
| DOI： |
| 分类号: |
| 基金项目:中德农业部科技合作项目(06-37);国家“十五”科技攻关项目（2004BA516A09） |
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| Optimizing for callus tissue culture system for the stem of Solanum tuberosum |
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| Abstract: |
| Four potato cultivars,Kexin No.12,Kexin No.13,Dongnong303 and Zaodabai were cultivated in vitro from stem explants.The results showed that the optimal medium of callus for Kexin No.12 and Kexin No.13 was MS+30 g/L sucrose+8g/L agar +2 mg/L 6-BA+1 mg/L 2,4-D;Dongnong 303 was MS+2 mg/L 6-BA +0.5 mg/L 2,4-D;the optimal medium of Zaodabai was MS+30 g/L sucrose+8 g/L agar+2 mg/L 6-BA+0.5 mg/L 2,4-D;the adventitious callus induction rate were 100%,100%,100% and 90%,respectively by using these optimal induced medium.The best medium for adventitious bud differentiation from stem callus was MS+30 g/L sucrose+8 g/L agar+4.0 mg/L 6-BA+0.1 mg/L NAA +1.0 mg/L GA3 for Kexin13;MS +30 g/L sucrose +8 g/L agar +2.0 mg/L 6-BA +0.5 mg/L NAA +1.0 mg/L GA3 for Kexin12;MS +30 g/L sucrose +8 g/L agar +2.0 mg/L 6-BA +0.1 mg/L NAA +1.5 mg/L GA3 for Dongnong 303;MS +30 g/L sucrose +8 g/L agar +2.0 mg/L +6-BA 0.5 mg/L NAA +1.0 mg/L GA3 for Zaodabai.Under these optimal induced medium,the rates of adventitious bud differentiation were 80%,90%,100%,and 76.7%,respectively. |
| Key words: potato,Kexin No.12,Kexin No.13,Dongnong303,Zaodabai,callus induction,callus differentiation |
