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外源Ca2+对水分胁迫下酿酒葡萄黑比诺主要抗旱生理指标的影响
惠竹梅1, 孙万金1, 张振文1
西北农林科技大学 葡萄酒学院
摘要:
为了探讨外源Ca2+对葡萄抗旱生理指标的影响,为Ca2+在葡萄耐旱生产中的应用提供参考依据,以盆栽酿酒葡萄黑比诺幼苗为试材,在水分胁迫条件下,用0.5,1.0和1.5 mmol/L CaCl2喷施黑比诺葡萄幼苗,以喷清水作为对照,研究水分胁迫下外源Ca2+对葡萄幼苗主要抗旱生理指标的影响。结果表明,与对照相比,CaCl2处理能够提高葡萄叶片可溶性糖含量、过氧化物酶(POD)及超氧化物歧化酶(SOD)活性,减缓叶绿素的分解,降低MDA含量。在整个水分胁迫期间,可溶性糖含量平均比对照提高46.4%~60.1%,SOD活性提高18.4%~66.6%,POD活性提高1.5%~2.5%,叶绿素a含量提高3.1%~9.4%,MDA含量降低8.1%~23.4%,CaCl2处理对叶绿素a的保护效果显著,对叶绿素b则无明显影响。以1.0 mmol/L CaCl2处理对提高黑比诺幼苗的抗旱性效果最佳。
关键词:  酿酒葡萄黑比诺  水分胁迫  抗旱生理指标
DOI:
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基金项目:农业部重点推广项目“黄土高原保护性耕作技术研究”;西北农林科技大学青年科研专项基金项目(06ZR048)
Effect of exogenous Ca2+ on drought resistance physiological indexes of wine grape cultivar Pinot Noir under water stress
Abstract:
To provide reference for applying exogenous Ca2+ in drought resistance viticulture,with wine grape cultivar Pinot Noir as experimental materials,the effect of Ca2+ on main drought resistance physiological indexes of grape seedling was studied in this article by spraying 0.5,1.0 and 1.5 mmol/L CaCl2 to seedling of Pinot Noir under water stress condition.Compared with spraying water(control),the result indicated that Ca2+ could increase soluble sugar content,peroxidase(POD) and catalase(SOD) enzyme activity,decrease MDA content,and alleviate chlorophyll decomposition.Compared with control during water stress process CaCl2 treatments increased soluble sugar content by 46.4%-60.1%,increased SOD and POD enzyme activity by 18.4%-66.6%and 1.5%-2.5% respectively,and increased the content of Chl.a by 3.1%-9.4%,while decreased MDA content by 8.1%-23.4%;effect of CaCl2 treatments on protection of Chl.a was obvious but ineffective to Chl.b.The effect of 1.0 mmol/L CaCl2 among the treatments was the best.
Key words:  wine grape cultivar Pinot Noir  CaCl2  water stress  drought resistance physiological index