| 摘要: |
| 人类人工染色体(HACs)由着丝粒、端粒和复制起点组成。通过对天然染色体的改造或者从头构建的方法可以获得多种类型HACs。HACs可以携带大片段基因组DNA,是建立转基因动物模型的重要手段,在基因治疗方面也有着广阔的应用前景。文章从HACs的结构及各组成元件的功能、HACs的不同构建方法、HACs在转基因研究及基因治疗中的应用等方面,对HACs的最新研究进展进行了综述,同时指出了HACs研究中存在的问题,并对HACs的应用前景进行了展望。 |
| 关键词: 人工染色体 转基因 生物反应器 基因功能 |
| DOI： |
| 分类号: |
| 基金项目:天津市农业科学院院长基金项目（06001） |
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| Advance in human artificial chromosome research |
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| Abstract: |
| Human artificial chromosomes(HACs) are composed of a functional kinetochor,a telomere and an origin of replication.Many kinds of HACs can be generated by two different approaches,either by fragmenting a natural chromosome or generating de nove chromosomes.HACs can carry large DNA fragments,which offer an importment way not only for producing transgenic animal model but also for gene therapy.The structural requirements for HACs,the approaches to constructing HACs,the applications of HACs in transgenic research and gene therapy are reviewed in this article.Technological problems and perspectives are also mentioned. |
| Key words: artificial chromosome,transgene,bioreactor,gene function |
