| 摘要: |
| 为了探讨金黄色葡萄球菌的氟喹诺酮耐药性与grlA、grlB基因突变的关系,从52株野生型金黄色葡萄球菌中筛选出1株对氟喹诺酮敏感的金黄色葡萄球菌,对其进行耐药诱导,获得一系列不同氟喹诺酮耐药水平的金黄色葡萄球菌,并对其grlA、grlB基因进行PCR扩增、测序及序列分析。结果表明,金黄色葡萄球菌的氟喹诺酮耐药性与grlB基因突变无关,而与grlA的丝氨酸(Ser)80→苯丙氨酸(Phe)和谷氨酸(Glu)84→赖氨酸(Lys)突变密切相关。金黄色葡萄球菌grlA的80位氨基酸和84位氨基酸双突变是其氟喹诺酮耐药性提高的标志之一。 |
| 关键词: 金黄色葡萄球菌 氟喹诺酮 耐药性 基因突变 |
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| 基金项目:国家自然科学基金项目（30170698）；吉林农业大学博士启动基金资助项目 |
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| Relation between fluoroquinolones-resistant and mutation of grlA and grlB gene in Staphylococcus aureus |
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| Abstract: |
| The purpose was to study the relation between fluoroquinolones-resistant and mutation of grlA and grlB gene in staphylococcus.aureus,the sensitive strain was induced with Ciprofloxacin to get grad resistance strain of S.aureus,the grlA and grlB gene were amplified and sequenced by polymerase chain reaction.Sequence analysis showed that grlB gene had no mutation,while Ser 80→Phe and Glu 84→Lys of the grlA gene happened.These assays demonstrated that Ser 80→Phe and Glu 84→Lys of the grlA mutation were the main mark of staphylococcus aureus to fluoroquinolones-resistant rising. |
| Key words: Staphylococcus aureus fluoroquinolone drug resistance grlA grlB gene mutation |
