| 摘要: |
| 为深入探索日本脑炎病毒（Japanese encephalitis virus，JEV）WHe株的致病机理，并为新型疫苗研发提供技术支持，采用长链RT-PCR技术对其基因组全长cDNA进行了扩增鉴定，并对含复杂二级结构的3′末端和5′末端非编码区扩增片段进行了测序分析。扩增结果表明，扩增出的JEV WHe株基因组全长cDNA分子近11kb大小；非编码区测序分析表明，扩增产物为WHe株所特有。提示长链RT-PCR法可用于JEV WHe株基因组全长cD—NA的扩增。 |
| 关键词: 日本脑炎病毒WHe株 全长cDNA 长链RT-PCR |
| DOI： |
| 分类号: |
| 基金项目:湖北省自然科学基金项目（2005ABA195） |
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| Amplification of full-length cDNA of Japanese encephalitis virus WHe strain by long RT-PCR |
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| Abstract: |
| In order to elucidate the pathogenesis of Japanese encephalitis virus (JEV) WHe strain and develope novel vaccine,the full-length cDNA was amplified by long reverse transcription-polymerase chain reaction（long RT-PCR）.The full-length cDNA was identified by PCR and the fragments which contain the 3′and 5′untranslation regions were cloned into pGEM-T vector,and the sequences of inserted fragments were determined by Invitrogen Corporation.The results showed that the approximate 11 kb full-length cDNAs of WHe strain were amplified by long RT-PCR and its correctness was proved by partial nucleotide sequence analysis.The genomic cDNAs of WHe strain were successfully amplified. |
| Key words: Japanese encephalitis virus WHe strain full-length cDNA long RT-PCR |
