| 摘要: |
| 为了探讨小鼠卵巢颗粒细胞体外生长的特点及增殖的调控因素，分别添加2 μg/mL胰岛素、50 ng/mL FSH和20 ng/mL EGF的培养液培养小鼠卵巢颗粒细胞，每隔24h对细胞进行计数，研究其对小鼠卵巢颗粒细胞增殖的影响。此外，还对培养过程中出现的卵巢颗粒细胞集落进行了AKP染色和C-kit免疫组化染色鉴定。结果显示，在培养的第4天添加EGF组和FSH组与对照组相比均具有显著的促增殖作用（P<0.05），添加胰岛素组促增殖作用不显著（P>0.05）；卵巢颗粒细胞集落经AKP染色和C-kit免疫组化染色均呈阳性。提示EGF和FSH对小鼠卵巢颗粒细胞具有促增殖效应，小鼠卵巢颗粒细胞具有干细胞的部分特性。 |
| 关键词: 卵巢颗粒细胞 体外培养 细胞增殖 小鼠 |
| DOI： |
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| 基金项目:陕西省自然科学基金项目（99SM66） |
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| Culture of mouse granulosa cells in vitro |
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| Abstract: |
| To investigate the growth characteristics and regulational factors contributed to proliferation of mouse granulosa cells in vitro,insulin (2 μg/mL),FSH (50 ng/mL) and EGF (20 ng/mL) were added into the basal medium,respectively.The cell number were counted every 24 hours,the effect of each factor on promoting mouse granulosa proliferation was researched.In addition,alkaline phosphatase (AKP) staining,and C-kit immunohistochemical staining were performed to identify the clones of granulosa cells appearing in culture.The results showed that the group added with EGF and FSH,but insulin (P>0.05),had significant effect on promoting proliferation(P<0.05) compared with the control group on the 4th day;the clones of granulosa cells were both positive for AKP and C-kit.Therefore,it was concluded that EGF and FSH could promote granulosa cells’proliferation and mouse granulosa cells had some properties of stem cells. |
| Key words: granulosa cells culture in vitro cell proliferation mouse |
