| 摘要: |
| 以野生梭梭带腋芽茎段为外植体进行离体再生研究，探讨不同植物生长调节剂、硝酸银和蔗糖的质量浓度对其愈伤组织诱导、不定芽分化和生根的影响。结果表明，野生梭梭带腋芽茎段愈伤组织诱导的最佳培养基为MS＋2，4-D 2.0 mg/L＋6-BA 0.5 mg/L＋蔗糖30g/L＋琼脂6.0 g/L，诱导率为100％；不定芽分化的最佳培养基为MS＋NAA 0.5 mg/L＋TDZ 1.0 mg/L＋AgN03 3.5 mg/L＋蔗糖26 g/L＋琼脂6.5 g/L，不定芽分化率为97.8％；影响野生梭梭再生的最主要因素是NAA，其次是TDZ和AgNO3，蔗糖影响最小。 |
| 关键词: 梭梭 茎段 离体培养 植株再生 |
| DOI： |
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| 基金项目:河南省重大科技攻关项目（0524050006） |
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| Plant regeneration in vitro from stem shoots explants of wild Haloxylon ammodendron |
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| Abstract: |
| The regeneration system of Wild Haloxylon ammodendron in vitro was studied with stem shoots as explants to investigate the effects of different plant growth regulators,AgNO3 and sucrose etc on H.ammodendron stem shoots callus induction,bud differentiation and rooting of explants.Results showed the optimal medium for callus induction was MS +2,4-D 2.0 mg/L +6-BA 0.5 mg/L sucrose 30 g/L agar 6.0 g/L,and the induction frequency was 100%;the best bud differentiation medium was MS +0.5 mg/L NAA +1.0 mg/L +TDZ 3.5 mg/L +AgNO3 26 g/L sucrose 6.5 g/L agar,and the bud differentiation rate was 97.8%;the major factors affecting the bud differentiation and bud multiplying of H.ammodendron plants were NAA and TDZ in the first place and AgNO3,and sucrose in the next place. |
| Key words: Haloxylon ammodendron stem shoots in vitro culture plant regeneration |
