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BTH诱导小麦对白粉病的抗性与几丁质酶和β-1,3-葡聚糖酶活性诱导的关系
陈 鹏1, 李振歧2
1.西北农林科技大学 植物保护学院;2.西北农林科技大学 植物保护学院
摘要:
为了探讨BTH对小麦白粉病抗性的诱导以及抗性的部分生理机制,分别用0.01,0.05,0.1,0.2,0.4,0.8和1.6 mmol/L苯并噻二唑(BTH)溶液处理小麦幼苗2 d后接种白粉病菌,结果表明,浓度大于0.2 mmol/L的BTH处理均能显著诱导小麦幼苗产生对白粉病的抗性;用0.4 mmol/L BTH处理小麦幼苗后间隔不同时间接种白粉病菌,表明BTH诱导小麦产生对白粉病的抗性持久期在7 d以上;对BTH处理或接种白粉病菌的幼苗几丁质酶和β-1,3-葡聚糖酶活性的测定结果表明,BTH处理可系统性地增强这2种酶的活性且与小麦对白粉病的诱导抗性密切相关。
关键词:  小麦白粉病  诱导抗性  几丁质酶  β-1,3-葡聚糖酶
DOI:
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基金项目:西北农林科技大学青年学术骨干支持计划项目;杨凌农业生物技术育种中心资助项目
Relationship between induced resistance to Blumeriagraminisf. sp.tritici by BTH and activities of chitinase and β-1,3-glucanase in wheat
Abstract:
Benzothiadiazole (BTH) induced resistance to Blumeriagraminisf.sp.tritici in wheat seedlings and physiology mechanism were studied.0.01,0.05,0.1,0.2,0.4,0.8 and 1.6 mmol/L BTH were sprayed on wheat seedling 2 days before inoculation with Blumeriagraminisf.sp.tritici.Results showed that concentration over 0.2 mmol/L BTH could induce resistance against powdery mildew.Expression of resistance in the leaves could last at least 7 days.Chitinase and β-1,3-glucanase activities analysis showed that induced resistance in wheat was accompanied by a systemic increase in both enzymes activities.Two enzymes were related to resistance to powdery mildew in wheat.
Key words:  wheat powdery mildew  BTH  induced resistance  chitinase  β-1,3-glucanase