| 摘要: |
| 为了探讨下丘脑对妊娠的调节作用，采用免疫组织化学SP法研究了妊娠中期，正常妊娠大鼠及流产大鼠下丘脑内IFN-γ阳性神经元的形态与分布，同时利用图像分析软件测定了其灰度值。结果显示，正常妊娠大鼠和流产大鼠IFN-γ主要在下丘脑的视前大细胞核、室旁核、视上核、下丘脑前核等部位分布，而流产大鼠的IFN-γ阳性细胞数多于正常妊娠组且表达强于正常妊娠组。表明IFN-γ在下丘脑的表达对妊娠大鼠的神经内分泌调节有重要影响。 |
| 关键词: 妊娠大鼠 下丘脑 IFN-γ 免疫组化 |
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| 基金项目:国家自然科学基金资助项目( 39670550,30070093 );西北农林科技大学科技创新基金 |
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| Expression of IFN-γ in hypothalamus from aborted rat |
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| Abstract: |
| To approach the regulation of hypothalamus on pregnancy,immunohistochemistry SP method was used to investigate the morphous and distribution of IFN-γ-positive-neurons in hypothalamus of normal or abortion rats’during mid trimester of pregnancy.The gray scale value was also determined by using image analysis software.The results showed that IFN-γ-positive-neurons were distributed mainly in pre-opticus magno cellularis nucleus,paraventricular nucleus,supraoptic nucleus,anterior hypothalamic nucleus in both normal pregnancy and aborted rats’hypothalamus.While the number of IFN-γ-positive-neuron in hypothalamus of aborted rats’was larger than in normal pregnancy ones.IFN-γ was also expressed more strongly in aborted rats’hypothalamus.It can be concluded that the expression of IFN-γ in hypothalamus plays an important role in neuroendocrine regulation during pregnancy |
| Key words: pregnant rat hypothalamus IFN-γ immunohistochemistry |
