| 摘要: |
| 为了研究不对称PCR-SSCP在基因突变检测中的准确性,以鲁西黄牛和荷斯坦奶牛为研究对象,用不对称PCR-SSCP分析技术分析了牛SMAD4基因3＇端非翻译区的碱基突变情况,比较了不对称PCR-SSCP和传统PCR-SSCP分析的优缺点。结果表明,鲁西黄牛和荷斯坦奶牛SMA4基因3＇端非翻译区有1个T碱基插入突变位点和1个G→A突变位点,其中G→A突变产生了1个HhaⅠ酶切位点;不对称PCR-SSCP和HhaⅠ酶切分析116头鲁西黄牛和75头荷斯坦奶牛群体G→A突变位点的频率完全一致,说明不对称PCR-SSCP不仅可以用于基因突变的检测,而且具有较高的准确性;不对称PCR-SSCP较传统PCR-SSCP的条带少且带型清晰、稳定性较高。以上结果表明,不对称PCR-SSCP可以用于基因突变的检测。 |
| 关键词: 不对称PCR-SSCP 基因突变 SMAD4 |
| DOI： |
| 分类号: |
| 基金项目:国家“863”计划项目（2002AA242011） |
|
| Application of asymmetric PCR-SSCP in gene mutation detecting |
|
|
| Abstract: |
| The advantage and disadvantage of asymmetric PCR-SSCP and traditional PCR-SSCP were compared to analyze the character and accurate rate in detecting mutation in this study.The mutations in 3′UTR region of SMAD4 gene were analyzed by asymmetric PCR-SSCP in Luxi cattle and Holstein cow and one insert T mutation and one G→A mutation were found in this region.The G→A mutation created a HhaⅠ enzyme digestion position and the frequency was studied by asymmetric PCR-SSCP and enzyme digestion in 116 Luxi cattle and 75 Holstein cows and got the same results.The asymmetric PCR-SSCP had a few bands,which were clearer and stabler than traditional PCR-SSCP.This indicated the asymmetric PCR-SSCP was suitable for mutations detecting. |
| Key words: asymmetric PCR-SSCP gene mutation SMAD4 asymmetric |
