| 摘要: |
| 为了研究不同启动子驱动的反义SAGP抑制内源ADP-Glc PPase小亚基编码基因表达的效果,创造适于外源基因表达的突变体,利用RT-PCR方法,由马铃薯块茎cDNA克隆获得ADP-Glc PPase小亚基编码基因(SAGP)的1 821 bp cDNA。核酸数据库检索和序列比对分析表明,已克隆的SAGP基因cDNA推测编码607个氨基酸组成的多肽,其中第312和411的天冬酰氨均突变为丝氨酸。以pCambia为基础,分别构建了CaMV35S和GBSSI块茎启动子驱动的小亚基cDNA反义结构植物表达载体。 |
| 关键词: 腺苷二磷酸葡萄糖焦磷酸化酶(ADP-Glc Ppase) 基因克隆 启动子 反义结构 表达载体 |
| DOI： |
| 分类号:C979;F323.89 |
| 基金项目:国家自然科学基金资助项目(30160010) |
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| Cloning of SAGP gene and constructing of antisense transformation vectors |
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IA Xun-hua ZHANG Yi-lie LEI Hong WANG Yi
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| Abstract: |
| To compare effect of expression repression antisense SAGP driven by two promoters and subsequently to create potato mutation of tuber starch deficiency,a full length of cDAN encoding small subunit of ADP-Glc PPase(SAGP) was cloned and sequenced through RT-PCR with tuber of potato cultivar Zheng 1011.The sequence BLAST and alignment showed that SAGP of the 1 821 bp cDNA encodes a deduced peptide composed of 607 amino acids,and two mutations,N312S and N411S,occurred on the pepetide.With known vector pCambia for plant transformation,two antisense recombinant vectors,pS-SAGP and pG-SAGP in which SAGP were driven by promoters of 35S and GBSSI respectively,were constructed. |
| Key words: ADP-Glc PPase,gene cloning,promoter,antisense structure,transformation vector |
