| 摘要: |
| 小鼠颈背部皮下注射D-半乳糖生理盐水溶液(125 mg/(kg.d))诱导其衰老,同时分别灌胃10,20和40 mg/(kg.d)玉米幼芽提取物(Extract of maize plumule,EMP),每日1次,连续处理42 d后,测定心肌组织超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)和一氧化氮(NO)的含量,并用透射电镜观察心肌细胞超微结构,研究EMP对D-半乳糖致衰老小鼠心肌自由基代谢和超微结构的影响。结果表明,与对照组相比,D-半乳糖能明显的降低小鼠心肌组织中的SOD、CAT和GSH-Px活性,提高MDA、NO含量;与衰老模型组比较,20 mg/kg EMP处理组小鼠心肌组织的SOD、CAT和GSH-Px活性均极显著升高,MDA和NO含量均极显著降低;与对照组相比,衰老模型组小鼠的心肌Z线变宽、模糊,肌原纤维断裂、溶解、凝集,线粒体肿胀、空泡化、嵴消失;20 mg/kg EMP处理组的以上病变均有明显的改善。这表明20 mg/kg的EMP具有增强衰老小鼠心肌抗氧化酶活性,改善心肌自由基代谢,保护心肌细胞超微结构的作用。 |
| 关键词: 玉米幼芽提取物 D-半乳糖 抗氧化酶 自由基代谢 |
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| 基金项目:西北农林科技大学校长专项基金项目（130709） |
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| Effect of extract of maize plumule on free radical metabolism and ultrastructure of myocardium in aging mice |
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| Abstract: |
| Effect of extract of maize plumule(EMP) on free radical metabolism and ultrastructure of myocardium in aging mice induced by D-galactose was investigated.Physiological saline solution of D-galactose was applied in subcutaneous injection on the back of neck 125 mg/kg per day,once daily continuously for 42 days to induce the aging model.At the same time,EMP(10,20 and 40 mg/kg) was given by gastric perfusion once daily continuously for 42 days too to study the activity of superoxide dismutase(SOD),Catalase(CAT),Glutathione peroxidase(GSH-Px) and the content of malondialdehyde(MDA) and nitric oxide(NO).Meanwhile,the ultrastructure of myocardium was observed under transmission electron microscope.The data show that D-galactose caused a significant decrease in the SOD,CAT and GSH-Px activity and increase in MDA and NO content versus control group.Compared with model group,mice treated with both D-galactose and EMP(20 mg/kg) obviously had a higher SOD,CAT and GSH-Px activity and lower MDA and NO content.Under transmission electron microscope,these pathological changes were observed in aging model group:Z-line broadening and illegibility;Myofilament fragmentation,dissolution,agglutination;Mitochondrion swelling,vacuolization,cristae vanishing.These pathological changes were significantly improved by EMP at a concentration of 20 mg/kg.The result indicates that 20 mg/kg EMP can increase the activity of anti-oxidation enzyme,improve free radical metabolism and protect ultrastructure of myocardium from oxidative stress in aging mice. |
| Key words: extract of maize plumule(EMP),D-galactose,anti-oxidation enzyme,free radical metabolism |
