| 摘要: |
| 以皇家嘎拉苹果叶片为材料提取总RNA，合成cDNA第一链后，先利用巢式PCR获得苹果脱氢抗坏血酸还原酶（DHAR）基因中间片段，再利用3′-RACE方法进一步获得3′末端核酸序列，共得到840 bp包含完整3′末端的DHAR基因cDNA片段，其序列与烟草、水稻、番茄中DHAR基因序列的同源性均大于70%，证明所克隆到的核苷酸片段确为苹果DHAR基因cDNA片段。 |
| 关键词: 苹果 脱氢抗坏血酸还原酶 克隆 3′-RACE 序列分析 |
| DOI： |
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| 基金项目:西北农林科技大学“拔尖人才支持计划”项目；教育部优秀青年教师资助计划项目(2097) |
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| Cloning and sequence analysis of cDNA segment encoding dehydroascorbate reductase from malus |
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| Abstract: |
| In this study,a cDNA fragment of dehydroascorbate reductase (DHAR) including whole 3'-end was cloned from Royal Gala by RT-(nest) PCR and 3′-RACE.As deduced from the 840 bp cDNA nucleotide,it presented more than 70% homologous traits with tobacco DHAR,Rice DHAR and tomato DHAR,These results suggest that the cDNA fragment is from DHAR gene of Royal Gala. |
| Key words: malus dehydroascorbate reductase (DHAR) cloning 3′-RACE sequence analysis |
